摘要
本文用单克隆抗体检测法和放射配体结合测定法研究了烫伤大鼠肝胞液免疫活性糖皮质激素受体(GR_(IR))和糖皮质激素受体结合容量(GR_B)的变化。结果显示,烫伤后GR_(IR)和GR_B均明显减少,且两者下降的趋势基本平行,但GR_B下降的幅度比GR_(IR)约高10%。这说明应激后GR_B的减少主要是GR蛋白分子减少所致,但约有10%则归因于结合活性的变化。作者对这些变化的可能机制进行了讨论。
Rats were immersed into boiling water for 15 seconds with scalded area about 23 cm^2 and decapitated at 0.5,1 and 6 hours after scalding. The changes of immunoreactive glueocorticoid receptor (GR_(IR)) and glucocorticoid receptor binding (GR_B) of hepatic cytosol after scalding were studied by both monoclonal antibody method and radioligand binding assay. The results showed that GR_(IR) and GR_B decreased markedly in parallel manner after scalding. Comparing with the controls (100%), the values of GRIR of 0.5,1 and 6 hours after scalding decreased to 64.76±13.320% (n=4),59.55±13.18% (n=5) and 42.64±4.24% (n=4)respectively; and relevent GR_B decreased to 50.24±9.71% (n=4), 51.11±11.31% (n=5) and 30.43±3.03% (n=4). The amplitude of decrease of GR_B was about 10% more than that of GR_(IR). Thus it might be concluded that the reduction of GR_B after stress was mainly due to the decrease of GR protein molecules but 10% of that was due to the change of GR binding activity. The mechanisms of these changes were discussed.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1992年第6期577-580,共4页
Chinese Journal of Pathophysiology
关键词
受体
糖皮质激素
鼠
烧伤
Receptors glucocorticoid
Antibody, monoclonal
Burns
Rats
