摘要
为了解铜、镉对泡泡草根、茎、叶细胞亚显微结构的毒害及作用位点 ,以期为进一步研究鸭跖草属植物的耐重金属机理提供理论基础 ,本文通过透射电子显微技术研究了 75 μmol·L-1铜和 5 0 μmol·L-1镉及其交互污染对泡泡草根、茎、叶细胞超微结构的影响和铜、镉在细胞中的分布 .结果表明 :75 μmol·L-1铜和 5 0 μmol·L-1镉均对泡泡草根细胞造成明显损伤 .铜使根细胞产生质壁分离、细胞质浓缩、部分细胞空泡化 ,使线粒体脊突消失、结构模糊、外膜破坏 ;镉使根细胞空泡化 ,并在部分空泡化的细胞里产生大小不等的颗粒状物 ;铜、镉交互污染使根细胞受害程度加深 ,并兼有两者的受害症状特征 :线粒体结构彻底破坏、空泡化细胞里的颗粒物更大电子密度更高、质壁分离现象更普遍、质膜上的颗粒物沉淀更大 .铜、镉及其交互污染使泡泡草茎细胞产生质壁分离 ,对叶细胞无明显伤害 .所以铜、镉及其交互作用在试验浓度下 ,对泡泡草各器官的损伤程度为根 >茎 >叶 .
A hydroponical experiment was carried out to study the toxicity of copper, cadmium and their combined pollution on ultrastructure of Commelina bengalensis Linn. Transmission Electron Microscope (TEM) was used to investigate the effect of heavy metal on ultrastructure of different parts of plants. Result showed: serious damage was found with Copper of 75?μmol·L^(-1) and Cadmium of 50?μmol·L^(-1) individually and also with both heavy metals present. Plasmolysis, concentrated cytoplasm, disappear ance of the cristae of mitochondria, ambiguity of framework and destruction of mitochondria envelope were all symptoms of root cells under Cu stress; hollowing cells was also found in Cd treatment, and there existed some different granule in these hollowing cells. Plasmolysis was found in all treatments in part cells of stem, but no damage was detected in cells of leaves. The damage degree to organs of Commelina bengalensis Linn was as follows: root>stem>leaves.
出处
《环境科学学报》
CAS
CSCD
北大核心
2004年第2期343-348,共6页
Acta Scientiae Circumstantiae
基金
国家自然科学基金 (40 12 5 0 0 5
4 0 2 710 6 0 )
国家重点基础研究发展规划 (2 0 0 2CB4 10 810 0 9)
