摘要
利用生理剂量碘作用于早期人胎大脑神经细胞无血清培养物,经离子交换法分离出神经细胞的神经原特异性烯醇化酶(NSE)和非神经原特异性烯醇化酶(NNE)。两酶的转换方式与体内相似,碘促进神经细胞 NNE(αα,αγ亚基)向 NSE(γγ亚基)转换。培养25天 KI(?)组NSE 含量比培养30天对照组含量高,培养30天 KI_2组 NSE 含量是对照组的3倍,且 NSE 出现提前,活性增高。培养15天时加碘的作用比开始培养时加碘的作用更加明显。
As physiological dose of iodine acts on the serum-free cultures of earlyhuman fetal cerebral neuron,non-neuronal enolase (NNE) and neuronspecific enolase (NSE) were seperated and analyzed for the effect of iodineon the expression of the latter in vitro cultivation.The results showed thatthe switch-over from NNE to NSE was similar to that iv vivo;NSE contentwas higher in DIV 25 culturc of KI_1 than DIV 30 control;NSE content of DIV30 of KI_2 was 2-fold high than control and also its activity was high.It wassuggested that iodine could promote the switch-over mechanism in neuronculture and the effect of iodine on NSE expression was more obvious whenthe neuron culture was added with iodine at the 15th day of iodine-freecultivation.
出处
《中国地方病学杂志》
CAS
CSCD
1992年第4期210-213,共4页
Chinese Jouranl of Endemiology
关键词
大脑神经细胞
碘化钾
NSE
胎儿
iodine
neuron specific enolase(NSE)
human fetus
in vitro
