摘要
目的 :探讨纯化的供鼠 NK细胞加入预处理方案与 MHC半相合骨髓移植后免疫耐受的关系。方法 :制备 (BAL B/ c H- 2 d× C5 7BL / 6 H- 2 b) CB6 F1 H- 2 d/b小鼠为受者 ,C5 7BL / 6 H- 2 b的小鼠为供者。 70只受者小鼠经致死剂量 (9.0Gy)照射后随机分为 7组 ,每组 1 0只。对照组 :分别为单纯照射组、MHC半相合 GVHD对照组、MHC半相合骨髓细胞移植组、同基因骨髓细胞移植组。实验组 :CB6 F1 H- 2 d/b小鼠在完成照射后 1 h内首先经静脉输注纯化后的MHC半相合供者 NK细胞 ,分为 1× 1 0 6、 5× 1 0 5和 2× 1 0 5/只 3个组 ,在照射后 4 h移植 MHC半相合骨髓细胞。以血像、体重、生存期和病理组织学等变化为观察指标并做组间比较。结果 :1生存期 :单纯照射对照组为 (5 .1 5± 0 .6 6 ) d,GVHD对照组为 (1 5 .80± 1 .93) d,其他组小鼠生存期均大于 30 d。 2病理学结果 ,MHC半相合 GVHD对照组出现典型的 GVHD病理表现 ,其他各组均无 GVHD病理表现。结论 :在 MHC半相合骨髓移植模型上证实 ,移植前将供鼠的纯化 NK细胞加入移植预处理方案 。
ObjectiveTo study the relationship of donor derived NK cells as pretreatment condition and immune tolerance after MHC haplotype mismatched BMT in mice. MethodsThe murine model of MHC haplotype mismatched BMT was established by using (BALB/c H 2d ×C57BL/6 H 2b ) CB6F 1 H 2d/b mouse as the recipient, and C57BL/6 H 2b mouse as the donor. Seventy recipient mice were divided into seven groups after irradiation (TBI 60 Co 9 0 Gy) . Control groups: simple irradiation group, MHC haplotype mismatched GVHD control group, MHC haplotype mismatched BMT group, syngeneic BMT group. Experimental groups: the mice were distributed into three groups of different doses of NK cells which were 1×10 6/one,5×10 5/one,2×10 5/one. CB6F1 H 2d/b mice were conditioned with 9 0 Gy, and the donor derived NK cells were injected into CB6F 1 H 2d/b mice, after a interval of four hours followed by infusion of C57BL/6 H 2b mice bone marrow cells. The effect was assessed by hemotopoietic reconstruction, survival time, body weight, histopathology in the recipients. Results①Life span: in the case of control group, survival time was (5 15±0 66) days for the simple irradiation group, survival time was (15 80±1 93)days for MHC haplotype mismatched GVHD control group, beyond 30 days for other groups. ②Pathological results: GVHD pathological manifestations were found in MHC haplotype mismatched GVHD control group. The effects in other cases assessed by histopathology indicated no GVHD. ConclusionThe results have shown that donor derived NK cells as pretreatment condition can induce immune tolerance after MHC haplotype mismatched BMT in mice.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2004年第2期188-190,F002,共4页
Journal of Jilin University：Medicine Edition
基金
全军医药卫生科研基金十五重点项目课题 (0 1 Z0 5 0 )
关键词
杀伤细胞
天然
骨髓移植
免疫耐受
kill cells,natural
bone marrow transplantation
immune tolerance
