摘要
目的检测耐Imipenem(IMP)的鲍曼不动杆菌(Imipenem-resistantAcinetobacterbaumannii,IMRAB)是否携带BalIMP基因以了解其耐药机制,分析IMRABs的耐药谱特点供临床治疗此类感染参考。方法K-B琼脂扩散法做抗生素敏感实验,PCR扩增BalIMP基因片段并测序及做BLAST分析。结果17株稳定耐受IMP的IMRABs有14株携带BalIMP基因,IMRABs对绝大多数常用β-内酰胺类抗生素耐药,对庆大霉素、丁胺卡那霉素及环丙沙星的敏感率为24.1%～34.5%。结论携带编码碳青霉烯酶的BalIMP基因是鲍曼不动杆菌耐受IMP的重要机制,应严格按照细菌学实验报告选择抗生素治疗此类感染.
Objective Detecting the BalIMP gene to find out the mechanism of the resistance to Imipenem of Imipenem-resistant Acinetobacter baumanniis,IMRAB, to analyse its drug resistance spectrum so to rationalize treatment. Method The fragment of BalIMP gene was amplified by polymerase chain reactions. Drug susceptibility was determined by K-B methods. Result There were 14 strains of the 17 strains IMRABs which resistance to Imipenem were stabile carrying the BlaIMP gene. IMRABs were resistant to most of beta-lactam antibiotics. The susceptibility rates of IMRABs to gentamicin,amikacin and ciprofloxacin were 24.1%, 34.5% and 34.5% respectively. Conclusion It is one of the most important mechanism carrying the BalIMP gene which coded the metallo-beta-lactamase (carbapene-mase) of the resistance against Imipenem of IMRABs.It is critical that the right antibiotics should be selected according to the results of microbiological tests strictly.
出处
《实用医药杂志》
2004年第3期238-240,共3页
Practical Journal of Medicine & Pharmacy
关键词
不动杆菌
耐药性
医院内感染
Acinetobacte spp. Drug resistance Nosocomial infections
