摘要
对花烛 (Anthuriumandraeanum)‘Jolanba’无菌苗叶片诱导产生的愈伤组织进行 3种不同的培养处理 ,观察其再分化表现 ,并探讨了 3种继代培养愈伤组织的生理生化特点。结果表明 ,适当降低基本培养基浓度、减少继代次数和延长继代周期培养的愈伤组织分化的芽较多、较壮 ;再生芽的分化需要较高水平的可溶性蛋白质 ,其生长需要较高水平的可溶性糖 ;POD和SOD活性与分化的芽数呈正相关 ;GA1+ 3 /ABA、ZRs/ABA的高比例与再生芽的高度呈显著正相关 ;IAA/ABA及IAA的高水平与花烛离体根的发生呈正相关。
Calli of Anthurium andraeanum‘Jolanba’derived from leaves of in vitro plantlets were cultured with three different treatments to observe their redifferentiation behaviors,and their physiological and biochemical features are discussed.The results went as followed.Decrease of base medium concentration,reduction of sub-culture frequency,or prolongation of sub-culture period favored differentiation and growth of regenerated plantlets.High content of soluble protein favored shoot differentiation,and high content of soluble sugar enhanced shoot growth.There was obvious positive correlation between activities of POD and SOD and number of differentiated shoots. So was between GA 1+3/ABA,as well as ZRs/ABA,and shoot height.The IAA/ABA ratio or IAA was also related to rhizogenesis.One of the physiological and biochemical causes for the enhancement growth vigor of aseptic plantlet by renewing in vitro culture system and prolonging culture period was maintaining high content of endogenous soluble sugar and favorable balance of endogenous phytohormes.
出处
《园艺学报》
CAS
CSCD
北大核心
2004年第1期69-72,共4页
Acta Horticulturae Sinica
基金
广东省科委资助项目 (K13 0 5 3 2 0 0 2 -1-4 )
江苏省教育厅高新技术产业化开发项目 (JH0 2 -0 86)
关键词
花烛
愈伤组织
继代培养
再分化
离体培养
Anthurium andraeanum
Redifferentiation
Physiological and biochemical features
Endogenous phytohormones
