摘要
作者报告建立和应用包括单克隆抗体结合免疫荧光、蛋白免疫印迹、DNA同源性杂交、DNA酶切图谱、脂肪酸气-质谱及免疫电镜在内的一套分子立克次体学方法,对我国蜱传斑点热立克次体病进行了分子流行病学研究。结果:(1)证明在新疆精河县、内蒙呼盟陈旗及北京昌平县存在北亚蜱传斑点热(北亚热)的自然疫源地;(2)在我国首次从北亚热病人中分离出西伯利亚立克次体(An-84,Se-85、W-88 3株),从草原革蜱(FT-84、MT-84 2株),中华革蜱(BJ-90 1株),草原革蜱蜱卵(TO-85 1株)也分离出同一性质立克次体,自易感人群、传播媒介和传染源3个环节中都找到了病原学证据;(3)在种的水平上研究了上述7株分离株和1株国内参考株(草原革蜱JH-74株),在分类学上确定了它们均属立克次体属、斑点热群、西伯利亚立克次体种(Rickettsia sibirica)。在我国东经80～135°,北纬40～50°广大地理范围内,用分子流行病学方法,确证和新发现了一个病种——北亚蜱传斑点热(North Asia Tick Borne Spotted Fever)。
We have isolated and identified the Rickettsia sibirica by monoclonal antibodies, protein immunoblotting, DNA homology hybridization, analysis of endonuclease restriction of chromosome DNA, gas chromatography and immunoelectromicroscopy. The north asia tick borne spotted fever (north asia fever) caused by R.sibirica was a newly found disease in China. The res- ults demonstrated that Jinghe county of Xinjing Uygur Autonomous Region, Chen-ba-er-hu-qi (county) of Inner Mongolia Autonomous Region and Changping county of Beijing are the natural foci of north asia tick borne spotted fever. This is the first time to isolate the spotted fever group (SFG) rickettisae from patients in China. We isolated the same species of SFG rickettsiae from Dermacentor nuttalli tick, tick ova and Dermacentor sinicus tick. Our results clearly showed the susceptible persons, vectors and reservoirs of the north asia fever in nature. All seven Chinese strains of SFG rickettsiae isolated by our laboratory and one Chinese reference strain of SFG rickettsiae belong to R.sibirica. The investigation with molecular epidemiologic methods demonstrated that a new disease, north asia fever widly distributed in northern China, which covered longitud 80～135east and latitud 40～50north.
基金
本课题为国家自然科学基金
关键词
分子流行病学
北亚蜱
斑点热
North asia tick borne spotted fever
Rickettsia sibirica
Molecular rickettsiology
Molecular epidemiology
