摘要
目的:研究leptin受体在人大肠癌细胞系及大肠腺癌组织中的表达,探讨与肿瘤血管生成、细胞增生之间的关系. 方法:免疫组织化学方法+图像分析检测leptin受体,CD34 及Ki67的表达,并计算肿瘤微血管密度及细胞增生指数. 结果:leptin受体在SW480,HT29细胞以及大肠癌组织均有表达,大肠腺癌组织的平均染色吸光度比正常大肠组织高(0.153±0.011 vs 0.115±0.071,P<0.05),与Ki67 指数呈正相关(r=0.388,P<0.05);血管内皮细胞表达leptin受体的大肠腺癌,其MVD较高(45.100±10.000vs 37.400±10.200,P<0.05),二者之间正相关(r=0.569, P<0.05);大肠腺癌MVD、Ki67指数比正常组织高(41.500±10.700 vs 31.300±11.100,P<0.01;0.458±0.108 vs 0.312±0.097,P<0.01);leptin受体的表达与大肠腺癌临床病理参数之间未见相关. 结论:leptin与大肠腺癌上的leptin受体结合后,在促进肿瘤细胞增生及肿瘤组织的血管增生方面具有一定的作用.
AIM: To investigation the expression of leptin receptor in colorectal cell lines and colorectal adenocarcinoma tissue, and the relationship between its expression, vascular proliferation and tumor cell proliferation. METHODS: The expression of leptin receptor, CD34 and Ki67 proteins was detected by immunohistochemistry in HT29 and SW480 cells and colorectal adenocarcinoma tissues. Leptin receptor, the MVD and cell proliferation exponent were analyzed by image analysis software. RESULTS: Leptin receptor was expressed in HT29 cells, SW480 cells and all colorectal adenocarcinoma tissue. The normal large intestine tissue also had leptin receptor, but the mean of their absorbance was lower than that of colorectal adenocarcinoma tissue (0.153±0.011 vs0.115± 0.071,P<0.05). The MVD and Ki67 exponent of colorectal adenocarcinoma tissue were both higher than those of normal controls (41.500±10.700 vs31.300±11.100, P<0.01; 0.458±0.108 vs0.312±0.097,P<0.01). There was a positive correlation between leptin receptor and Ki67 exponent (r =0.388, P<0.05). The MVD of colorectal adenocarcinoma tissue which leptin receptor expressed in blood vessel endothelial cells was higher than the negative ones (45.100±10.000 vs 37.400±10.200, P<0.05). The correlation could not be found between leptin receptor and clinical pathologic parameters of colorectal adenocarcinoma. CONCLUSION: Leptin may have some roles to accelerate tumor cell proliferation and vascular proliferation in colorectal adenocarcinoma tissue after it binds leptin receptor.
出处
《世界华人消化杂志》
CAS
2004年第3期585-589,共5页
World Chinese Journal of Digestology
