摘要
目的:研究CTLA4Ig基因在移植小肠局部表达及其表达产物对急性排斥反应的治疗作用. 方法:建立SD→Wistar的大鼠异位小肠移植模型,并随机分为实验组(CTLA4Ig转基因组)和对照组(非转基因组). 实验组供肠移植前经肠系膜上动脉注入脂质体包裹的CTLA4Ig cDNA,术后应用免疫组织学检查移植小肠中CTLA4Ig转基因产物的表达.移植术后3,7,10 d分别获取各组的移植小肠进行组织学检查及细胞凋亡测定. 结果:经CTLA4Ig cDNA处理的小肠在移植术后可见大量的CTLA4Ig表达.对照组移植肠在术后7,10 d分别出现I, Ⅱ度急性排斥反应,同时凋亡细胞数量显著增加.实验组移植肠术后未见排斥反应的病理学证据,凋亡细胞偶见. 结论:CTLA4Ig基因可在移植小肠局部转染表达,其表达产物可防止移植术后急性排斥反应的发生.
AIM: To evaluate the local expression of CTLA4Ig gene in small bowels and its action on preventing acute rejection of the small bowel allografts. METHODS: Wistar rats underwent heterotopic small bowel transplantation from SD rats. The recipients were divided into experimental group (allografts were transfected with CTLA4Ig gene) and control group (CTLA4Ig gene not transfected) randomly. In the experimental group, the donor small bowels were perfused In vitro with CTLA4Ig cDNA packaged with lipofectin vector via intra-superior mesen-teric artery before transplantation, and the CTLA4Ig expression in the small bowel grafts post-transplantation was assessed by immunohistology. On days 3, 7 and 10 post-transplantation, the allografts in each group were harvested for the examination of histology and assay of apoptosis. RESULTS: Small bowel allografts treated with CTLA4Ig cDNA showed abundant CTLA4Ig expression after transplantation. Acute rejection grade I on day 7 and grade Ⅱ on day 10 after transplantation was noticed in the control allografts, and a dramatically increased number of apoptotic enterocytes in parallel to the progressive rejection could be recognized. In contrast, the allografts treated with CTLA4Ig cDNA showed nonspecific histological changes and only a few of apoptotic enterocytes were found after transplantation. CONCLUSION: Local CTLA4Ig gene transfection of small bowel allograft is feasible, and the local CTLA4Ig expression in the allograft can prevent acute rejection after transplantation.
出处
《世界华人消化杂志》
CAS
2004年第3期685-688,共4页
World Chinese Journal of Digestology
基金
南京医科大学创新基金资助项目
No.200106~~
