摘要
采用两种电泳模式——毛细管区带电泳(CZE)和毛细管胶束电泳(MEKC)分别对山豆根中的生物碱进行分离分析.在优化的分离条件下,两种模式都以盐酸麻黄碱作内标物,在电压为25 kV和检测波长为208 nm的条件下进行.在CZE模式中, 以0.04 mol/L含.50％甲醇(V/V)的柠檬酸作为缓冲溶液(pH=4.5),能够分离得到6种生物碱;在MEKC模式中,以20 mmol/L硼砂溶液、30 mmol/L十二烷基硫酸钠(SDS)、40％甲醇(V/V)作为电解质溶液,能够分离得到7种生物碱.两种模式在所选定条件下,都能将山豆根中主要生物碱进行基线分离,且有较好的重现性和较低的灵敏度.在两种不同的分离模式下,分别对山豆根中的苦参碱和氧化苦参碱进行了定性定量研究,从分子结构上阐明了这两种主要生物碱在不同的分离模式下的迁移机制.
Both methods CZE and MEKC have been used to separate and analyse alkaloids in sophora tonkinensis gapnep, and the ephedrinehydrochloride has been selected as an internal standard. The capillary tempreture is controlled at 25 t, the applied voltage 25 kV and the detection wavelength 208 nm. Six and seven alkaloids are separated by CZE and MEKC respectively. The running buffer is composed of 0. 04 mol/L Citric acid, 50% Methanol(pH =4. 5) in CZE and 20mmol/L Na2B4O7 · 10H2O, 30 mmol/L sodium dodecyl sulfate(SDS), and 40% methanol in MEKC. The two methods show a good repeatability and low detection limit. The separation mechanism of two alkaloids is briefly discussed.
出处
《宁夏工程技术》
CAS
2004年第1期46-47,50,共3页
Ningxia Engineering Technology
基金
宁夏回族自治区科技攻关资助项目(2001-019-13)
