期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

BINDING AND INTERACTION OF TRANSCRIPTIONAL REGULATOR HUMAN DAXX WITH ADENOVIRUS12 E1B 55 KILODALTON ONCOPROTEIN 被引量：2

BINDING AND INTERACTION OF TRANSCRIPTIONAL REGULATOR HUMAN DAXX WITH ADENOVIRUS12 E1B 55 KILODALTON ONCOPROTEIN
下载PDF
导出
摘要 Objective: To study the disruption of co- localization of human Daxx(hDaxx) with promyelocytic leukemia protein(PML) at the PML oncogenic domains (PODs) by the interaction of hDaxx with adenovirus(Ad) 12 E1B 55 Kilodalton Oncoprotein (Ad12 E1B 55kD). Methods: The direct binding reaction of hDaxx and Ad12 E1B 55kD was analyzed by coimmunoprecipitation and Western blotting in vivo or in vitro. The interaction of hDaxx with Ad12 E1B 55kD was studied using yeast two-hybrid assay. Results: hDaxx bounded directly to Ad12 E1B 55kD in vivo and in vitro. hDaxx interacted with full length Ad12 E1B 55kD. Conclusion: Transcriptional regulator hDaxx directly binds to and interacts with Ad12 E1B 55kD. Objective: To study the disruption of co- localization of human Daxx(hDaxx) with promyelocytic leukemia protein(PML) at the PML oncogenic domains (PODs) by the interaction of hDaxx with adenovirus(Ad) 12 E1B 55 Kilodalton Oncoprotein (Ad12 E1B 55kD). Methods: The direct binding reaction of hDaxx and Ad12 E1B 55kD was analyzed by coimmunoprecipitation and Western blotting in vivo or in vitro. The interaction of hDaxx with Ad12 E1B 55kD was studied using yeast two-hybrid assay. Results: hDaxx bounded directly to Ad12 E1B 55kD in vivo and in vitro. hDaxx interacted with full length Ad12 E1B 55kD. Conclusion: Transcriptional regulator hDaxx directly binds to and interacts with Ad12 E1B 55kD.
作者 万艳平 吴移谋 朱翠明 谭立志 余敏君 廖端芳
机构地区 Department of Microbiology Department of Pharmacology
出处 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2003年第4期273-276,共4页 中国癌症研究（英文版）
基金 This work was supported by grants from Ministry of Education of P.R. China (No. 2000-65).
关键词 Human Daxx Ad12 E1B 55kD BINDING INTERACTION Human Daxx Ad12 E1B 55kD Binding Interaction
分类号 R73-3 [医药卫生—肿瘤]
  • 相关文献

参考文献2

二级参考文献13

  • 1[1]Yang, X., L., Khosravi-Far, R., Chang, H., Y., et al. Daxx, a novel Fas-binding protein that activates JNK and apopotosis[J].Cell, 1997,89:1067 ～ 1076.
  • 2[2]Ishov, M., A., Sotnikov, G., A., Negorev, D., et al. PML is critical for ND10 formation and recruits the PML-interacting protein Daxx to this nuclear structure when modified by SUMO-1 [J].J. Cell Biology, 1999,147: 221 ～ 233.
  • 3[3]Pluta, A.,F., Earnshaw, W., C. & Goldberg, I., G. Interphase specific association of intrinsic centromere protein CENP-C with Hdaxx, a death domain-binding protein implicated in Fas-mediated cell death[ J ]. J. Cell Science, 1998,111: 2029 ～ 2041.
  • 4[4]Liu, y., Colosimo, A.L., Yang, X.J., et al. Adenovirus E1B 55-kilodalton oncoprotein inhibits p53 acetylation by PCAF[J].Mol. & Cell. Bio.2000,20:5540～5553.
  • 5[5]Sternsdorf,T.,Grotzinger,T.,Jensen,K.,et al. Nuclear dots: actors on many stages[J]. Immunobiology, 1997,198: 307 ～ 331.
  • 6[6]Kaelin, W. G. The emerging p53 gene family J [J]. Natl Cancer Inst, 1999,91:594～598.
  • 7[7]Kiriakidou, M., Driscoll, D. A., Lopez-Guisa, J. M., et al.Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region[J ]. DNA and Cell Bio, 1997,16:1289～ 1298.
  • 8[8]Li, H., Leo, C., Zhu, J., et al. Sequestration and inhibition of Daxx-mediated transcriptional repression by PML [J]. Molecular and cellular biology, 2000,20:1784～ 1796.
  • 9[1]Wienzek, S., Roth, J. and Dobbelstein, M. E1B 55-kilodalton oncoproteins of adenovirus types 5 and 12 inactivate and relocalize p53, but not p51 or p73, and cooperate with EAorf6 proteins to destabilize p53[J]. J. Virol., 2000,74:193～202.
  • 10[2]Punga, T. and Akusjarvi, G. The adenovirus-2 E1B-55K protein interacts with a mSin3A/histone deacetylase 1 complex[J]. FEBS Letters, 2000,476:248～252.

共引文献11

同被引文献2

引证文献2

Chinese Journal of Cancer Research
2003年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部