摘要
目的 通过半导体锥型散射激光热消融肝组织的实验研究 ,探索一项价廉、疗效好、应用方便的有自主知识产权 (试验阶段 )的热消融肝癌新技术。 方法 应用自我研制的实体肿瘤锥形散射激光治疗系统热消融健康家兔的肝组织 ,比较消融前后肝功能变化 ,观察组织温度变化及消融 1个月后的病理变化。 结果 术后家兔均成活 ,热凝固 10min光纤中心、距光纤中心 5mm、10mm处肝组织温度分别为 96 39℃± 3 97℃、6 0 79℃± 6 2 1℃、4 6 10℃± 4 5 8℃ ;术后 7d肝功能与治疗前比较无显著差别 (P >0 0 5 )。术后 1个月可见消融区横径为 2 6 0mm(2 1mm～ 33mm)灰白色凝固坏死带和纤维包膜。光镜下可见中心为凝固坏死 ,外周为 0 5mm炎症细胞浸润带和 2 4mm肉芽组织。 结论 半导体锥形散射激光可安全有效热消融肝组织 ,提示该技术有可能成为肝癌热消融治疗的一种适合国情的新方法。
Objective To seek a safe, efficient, and cost-effective technique for local thermo-ablation of hepatic cancer. Methods The livers from 16 healthy rabbits were thermocoagulated by diode-laser with scanner fiber tip, 6 w for 10 mins. At the same time, the temperatures were measured at 0, 5 and 10 mm from laser tip. The pre-thermocoagulative liver function was compared with that of 7 days post-thermocoagulation. The pathologic changes were also observed 1 month after laser thermocoagulation. Results All the rabbits survived and hepatic tissue temperatures at 0, 5, 10 mm from laser tip reached 96.39℃±3.97℃, 60.79℃±6.21℃, 46.10℃±4.58℃ respectively after 10 minutes of thermocoagulation. There were no significant differences in liver function parameters between rabbits of pre-laser thermocoagulation and of post-laser thermocoagulation. Thermocoagulated necrosis of liver tissue with surrounding fibrosis in a diameter of 26.0 mm was formed. Light microscopy revealed coagulative necrosis in the center of the coagulated area without surviving hepatic cells. Conclusion The hepatic tissue can be coagulated safely and effectively by diode-laser with scanner fibertip, and such a technique may provide a new method for the treatment of hepatic carcinoma.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2003年第11期849-851,共3页
Chinese Journal of Surgery
基金
国家863激光技术青年科学基金资助项目(86341020015)
