摘要
目的 研究红霉素刺激下对人胸膜间皮细胞分泌肿瘤坏死因子α (TNF α)、转化生长因子 β1(TGF β1)和缝隙连接蛋白 4 3(connexin 4 3)表达的影响 ,探讨胸膜粘连机制。方法 通过体外培养人胸膜间皮细胞 (HPMC) ,采用ELISA法测定不同浓度红霉素刺激下培养上清液TNF α、TGF β1浓度变化 ;Western印迹法测定红霉素对HPMC的缝隙连接蛋白 4 3水平的影响。结果 10 0mg/L的红霉素与HPMC一起孵育 3或 5d后可引起HPMC分泌的TNF α增多 ;不同浓度红霉素和孵育时间下HPMC分泌TGF β1明显升高 ;10 0mg/L红霉素刺激下HPMC的缝隙连接蛋白 4 3的水平下降 ,与作用的时间长短无关。结论 红霉素能够刺激HPMC分泌TNF α和TGF β1,可能是红霉素胸膜固定术的作用机制之一。红霉素改变HPMC的缝隙连接蛋白 4 3水平 ,可能是HPMC对刺激的反应和功能调节。本实验的各项观察指标多在较高浓度的红霉素刺激下发生变化 ,提示临床上应用红霉素作胸膜粘连应以浓度较高的溶液为宜。
Objectives To investigate the effects of erythromycin on secretion of tumor necrosis factor-α (TNF-α) and transforming growth factor-β_1 (TGF-β_1), and the level of connexin 43 in human pleural mesothelial cells(HPMC), and to explore the mechanism of pleurodesis. Methods HPMC was incubated with erythromycin at concentrations of 100 mg/L or 25 mg/L. The levels of TNF-α and TGF-β_1 in the supernatants were measured by ELISA method, and levels of connexin 43 were detected by Western blot. Results The secretion of TNF-α by HMPC increased after incubation with 100 mg/L erythromycin for 3 or 5 days,and the secretion of TGF-β_1 increased markedly after incubation with lower or higher concentrations of erythromycin. The levels of connexin 43 in HPMC decreased after stimulation with 100 mg/L erythromycin, but no relationship was observed between the levels and the stimulation time. Conclusions HPMC incubated with erythromycin showed increased secretion of TNF-α and TGF-β_1, which may be one of the mechanisms for erythromycin pleurodesis. Erythromycin decreased the level of connexin 43 in HPMC, and this effect may be a response of the cells to the stimulus.A high concentration of erythromycin is suggested for clinical pleurodesis based on the results from this study.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2003年第4期206-209,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
广东省自然科学基金 ( 960 74)
