摘要
目的 :探讨益气活血方对气虚血瘀证局灶性脑缺血再灌注损伤大鼠Fas、FasL蛋白表达的影响。方法 :采用气虚血瘀证局灶性脑缺血再灌注损伤大鼠模型 ,用线栓法阻塞大鼠大脑中动脉复制局部脑缺血再灌注模型 ,缺血 2h后再灌注 1 ,3,7d。用免疫组化染色法分别检测缺血皮质Fas、FasL蛋白表达。结果 :与模型组比较 ,益气活血方组Fas、FasL蛋白表达显著降低。结论 :益气活血方可能通过抑制Fas、FasL蛋白表达 ,减轻气虚血瘀证局灶性脑缺血再灌注损伤。
Objective:To investigate the effects of Qi invigorating and blood activating therapy on Fas and FasL protein expression in rats with Qi deficiency and blood stasis following local cerebral ischemia reperfusion. Method: The model with Qi deficiency and blood stasis syndrome following local cerebral ischemia reperfusion was employed, the middle cerebral arteries of SD rats were occluded for 2 hours and reperfused for 1,3, 7 days, respectively. Fas and FasL protein expression were detected by immunohistochemistry method. Result: In the Qi invigorating and Blood activating group, Fas and FasL protein expression decreased obviously. Conclusion: Qi invigorating and Blood activating therapy could alleviate local cerebral ischemia reperfusion injury in rats with Qi deficiency and blood stasis syndrome by inhibiting the expression of Fas and FasL protein.
出处
《安徽中医学院学报》
CAS
2004年第2期35-37,共3页
Journal of Anhui Traditional Chinese Medical College
基金
安徽省自然科学基金资助项目 (0 0 0 4 4 1 1 6 )
