摘要
目的 :探讨附子生物碱提取的影响因素 ,确定适宜的提取条件。方法 :以药典法测定提取物收率 ,以分光光度法测定生物碱含量 ,采用单因素试验法 ,对可能影响附子生物碱提取的因素分别进行考察。结果 :提取溶剂以 85%乙醇为宜 ,采用药材中粉作为提取原料 ,加 10倍量的提取溶剂提取 2次 ,每次 1.75h。以此条件制得的提取物收率为 1.2 1% ,生物碱含量为 0 .3 3 %。结论
Objective: To determine the suitable condi tions for extracting alkaloidals from Aconitum carmichaili.Method: Using methods in Chinese Pharmacopeia for dete rmining yields of extraction, which include spectrometry and single-factor tes t s, we investigated vavious factors that would affect the extraction of alkaloid s from Aconiti. Result: The suitable extractant was 85% ethanol, starting material was medium- size powder of aconiti, 10 times of the volume of the solvent was used in extr a ction for twice 1.75 h each time. The extraction yield was 1.21%, with a conte nt of 0.33% alkaloids. Conclusion: Extraction conditions determined with single factor test was consistent and feasible.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第3期222-224,共3页
China Journal of Chinese Materia Medica
