摘要
本文探索了用抗 CALLA 和抗 TNF 的双功能抗体导向 TNF 杀伤 CALLA 阳性白血病细胞的可行性.用化学交联剂 SPDP 制备了抗 TNF 和抗 CALLA 抗体的交联物,经鉴定为双功能抗体;与 TNF 联合使用,在 CALLA 阳性白血病细胞系 nalm—6上进行体外杀伤实验。MTT 比色法测定杀伤效果。我们发现经双功能抗体导向后,nalm—6细胞对 TNF 的敏感性明显增加。
This work probes the practicability of targetting TNF to kill CALLA positive Leukemia cell line by bifunc-
tional antibodies.Con jugate of anti—TNF and anti—CALLA antibody is acquired by chemical crosslinking.The
conjugate are identified as bifunctional antibodies and used together with TNF to in vitro kill CALLA positive
leukemia cell line nalm—6;the result is tested by colorimetric MTT assay.It is found that the sensitivity of nalm
—6 cells to TNF increases obviously because of bifunctional antibodies targetting.
Bifunctional antibody and cytokine are thought by this work to be practical and fascinating for immunotherapy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第6期341-344,共4页
Chinese Journal of Immunology
关键词
双功能抗体
肿瘤
血白病
免疫疗法
bifunctional antibody
BFA
also bispecific antibody
BsAb
tumor necrosis factor
TNF
common acute lymphoyte leukemia antigen
CALLA
immunotherapy
