摘要
目的 探讨大鼠脑缺血再灌流后神经元损伤的机制以及藻蓝蛋白对神经元的保护作用。方法 用线栓法制作大鼠左侧大脑中动脉阻塞再灌流模型 (MCAO) ,分别对假手术组、模型对照组、藻蓝蛋白组进行神经功能缺失评分 ,用TTC染色法测量脑梗死体积 ,用干湿重法计算脑的含水量 ,应用原位末端标记 (TUNEL)观察脑缺血再灌流不同时间点神经元凋亡的变化。结果 (1)在大鼠脑缺血再灌流后 2小时应用藻蓝蛋白 ,能够明显改善神经功能缺失症状 ,脑缺血再灌流后 2 4小时 ,藻蓝蛋白组的Bederson神经功能评分与模型对照组间差别有显著性意义 (P <0 0 5 ) ,且这种差异一直持续到脑缺血再灌流后 4 8小时。藻蓝蛋白还能够缩小脑梗死的体积 ,脑缺血再灌流后 4 8小时 ,藻蓝蛋白组的脑梗死体积与模型对照组间差别有显著性意义 (P <0 0 5 )。藻蓝蛋白还能够减轻脑水肿 ,使脑组织的含水量明显减少。 (2 )模型对照组 ,脑缺血再灌流后凋亡神经元主要分布于缺血周围区 ,随着再灌流时间的延长 ,凋亡细胞数逐渐增加 ,至 2 4小时达高峰 ,2天开始下降 ,14天时与假手术组间差别仍有显著性意义 (P <0 0 5 )。藻蓝蛋白组凋亡细胞的数量相对较少 ,其变化规律与模型对照组相似 ,同一时间点相比较 。
Objective To study the protection of phycocyanin on the neurons after focal cerebral ischemia/reperfusion in rats.Methods A rat middle cerebral artery occlusion/reperfusion (MCAO) model was produced using the intraluminal filament method.The rats were divided into three groups:sham operation group,model control group and phycocyanin group.After MCAO,the neurobehavioral testing of all rats was made.The infarction area was evaluated with the method of 2,3,7-triphenyltetrazolium chloride (TTC) staining.The H 2O% of the brain was calculated with the dry-wet method.The numbers and anatomic distribution of apoptotic cells were examined with terminal deoxynucleotidyl tranferase mediated dUTP-flourescein nick end-labeling (TUNEL) assay.Results (1) After focal cerebral ischemia/reperfusion in rats,phycocyanin can improve the deficits of the cerebral,decrease the infarction area and reduce the H 2O% of the brain.(2) In the model control group,TUNEL-positive cells were preferentially located in the penumbral area and progressively increased up to and peaked at 24h after reperfusion,and then decreased at 2d,but still in high level.In the phycocyanin group,TUNEL-positive cells were also mainly in the penumbral area,but the number of the cells were significantly low than the number of the model control group.The time-phase pattern of TUNEL-positive cells is similar to the pattern of the model control group.Conclusion These results suggest that the neuronal apoptosis is a dynamic ongoing process.Phycocyanin might play an important role in the protection of ischemic neurons.
出处
《中国全科医学》
CAS
CSCD
2004年第8期527-530,共4页
Chinese General Practice
基金
山东省自然科学基金资助项目
Y2 0 0 1C0 4
