摘要
水稻品种叶瘟鉴定,先后已应用了喷雾、注射、钳叶、针刺、涂抹等多种接种方法,鉴定效果良好。但在自然状态下采用注射、喷雾、湿棉球包穗等接种方法鉴定穗瘟,还遇到品种间抽穗期不一致和接种条件难以控制的难题。因此,目前常以诱发叶瘟的抗性来评价品种抗瘟性。叶瘟和穗瘟抗性虽有较高的相关性,但也存在一定的差异,使叶瘟鉴定结果并不能完全反映穗瘟抗生。但品种抗性的评价以穗瘟抗性最为重要,因此,研究穗瘟抗性鉴定的简易技术十分必要。
A technique for inoculating with Pyricularia oryzae Cav. for neck blast resistance screening was studied. Inoculation is carried out by spraying or smearing the mixture 1-2×10~5 conidia/ml suspended in 2% carboxymethyl cellulose (CMC) onto the in vitro rice panicle with a brush. The new method is suita- le for inoculation at 0-10 days after heading, especially at the 0-3 days after heading. The disease index af- ter inoculation is injection > smearing > spraying two times > spraying one times > around neck with cot- ton soaked in spore suspension. The method requires only a small amount of spore suspension and gives a high infection frequency, when smearing inoculation was used. It is an effective method for studying the re- sistance resource screening and resistance inheritance of neck blast.
出处
《中国水稻科学》
CAS
CSCD
北大核心
1992年第1期39-42,共4页
Chinese Journal of Rice Science
关键词
人工接种
离体稻穗
穗颈瘟
稻瘟病
Artificial inoculation
In vitro rice panicle
Neck blast
Pyricularia oryzae
