摘要
目的 :通过研究三叉神经痛患者疼痛发作时是否有降钙素基因相关肽 (calcitoningene -relatedpeptide ,CGRP)参与 ,痛支与非痛支神经组织中CGRP含量是否有所不同 ,加深对三叉神经痛发病机理的认识。方法 :用放射免疫法检测 16例三叉神经痛患者疼痛发作时患侧颈外静脉血、肘静脉血及术后患侧颈外静脉血中CGRP含量并以 10名同年龄组健康成人颈外静脉血中的CGRP含量作为正常对照 ;用免疫组织化学法标记 16例患者痛支与非痛支神经切片中CGRP免疫反应阳性颗粒 ,定量观察痛支与非痛支神经纤维中CGRP免疫反应阳性颗粒的数量、面积、平均光密度和平均面积 ,从免疫反应阳性颗粒的数量和面积两个方面来说明痛支与非痛支神经纤维中CGRP的含量有无差异 ;结果 :疼痛发作时患侧颈外静脉血中CGRP含量显著升高 ,与肘静脉血、术后患侧颈外静脉血及健康对照组颈外静脉血中的CGRP含量相比 ,差异非常显著 (P <0 .0 1) ,而后三者相比差异均不显著 (P >0 .0 5 ) ;痛支神经纤维中CGRP免疫反应阳性颗粒的数量、面积均明显高于非痛支中CGRP免疫反应阳性颗粒 ,差异非常显著 (P <0 .0 1) ;结论 :CGRP与三叉神经痛发病关系密切 ,三叉神经痛发作时局部确有CGRP的参与 。
Objective: To observe whether calcitonin gene-related peptide(CGRP)participates in the attack of trigeminal neuralgia, get a deeper understanding about its pathogenetic mechanism. Methods: During the attacks of trigeminal neuralgia, CGRP levels of plasma both the external jugular and cubital vein blood ipsilateral to pain were assessed by sensitive radioimmunoassay in 16 cases, after operations the venous blood from the external jugular vein was sampled and assessed again, the external jugular vein blood of 10 normal volunteers was collected as control. CGRP-like immunoactive granules of suffering and normal branches in 16 cases were labelled using ABC immunohistochemistry methods. Quantitative morphometry was performed using HPIAS-1000 high definition color morphometry system, the mean opaque density (MOD), positive unit (PU) and actual area (AA) were compared with eath other. Results: During the attacks of trigeminal neuralgia the levels of CGRP in the external jugular vein blood were significantly highter than that in the cubital fossa vein blood, postoperative external jugular vein blood and the external jugular vein blood of normal volunteers (P<0.01) . The number and area of CGRP-like immunoreactive positive granules in the pain branches was much more and larger than that in the painless branches. Conclusion: There are some relationship between CGRP and trigeminal neuralgia, CGRP indeed participate locally in the attack of trigeminal neuralgia, the rise of CGRP levels may decrease the threshold of pain in the local region, It may promote the release of Substance P in central nerve system (CNS) and enhance the neurogenic inflammation of Substance P in peripheral nerve terminals.
出处
《口腔医学研究》
CAS
CSCD
2004年第2期173-176,共4页
Journal of Oral Science Research
