摘要
目的 探讨不同剂量的DLF对小鼠肝脏的保护作用。方法 先用DLF预防给药 ,再采用CCL4 制作小鼠急性化学性肝损伤模型 ,以BDD为阳性对照。采用全自动生化分析仪测定血清ALT、ALP、AST和LDH的含量 ;联苯三酚自氧化法测定血清SOD含量 ;TBA显色法测定肝匀浆中的MDA含量 ;肝组织常规制片 ,组织学观察。结果 不同剂量的DLF组血清ALT、ALP、AST、LDH和肝组织中MDA含量明显低于CCL4 组 ,血清SOD组明显高于CCL4 组 ;镜下病理显示 ,DLF对由CCL4 引起的小鼠急性化学性肝损伤造成的肝细胞变性、坏死有一定的保护作用。结论 DLF对由CCL4 引起的小鼠急性化学性肝损伤有保护作用 ,其作用机制可能与其抗自由基有关。
Objective To Study the protective effects and the mechanism of DLF extraction on liver injury caused by CCL 4. Method DLF of different doses (0.5g/kg, 1.0g/kg and 2.0g/kg) were injected to the mice for ten days. Then CCL 4 was given on the last day. After 15 hours, the blood and livers of the mice were collected. The enzyme activities of ALT, ALP, AST and LDH in serum were measured by automatic biochemistry analyzer. The activity of SOD in serum was measured by self-oxidation of pyrogallic acid. The content of MDA in liver homogenate was measured by TBA colorimetric assay. The pathological changes in liver tissue were observed.Results Compared with the group of CCL 4, DLF not only decreased the activities of ALT, ALP, AST and LDH in serum and MDA in liver tissue, but also increased the production of SOD activity in serum. The pathological section shows that DLF has protective effect on mice, which suffer liver injury from CCL 4.Conclusion The DLF can protect mice from acute chemical liver injury by CCL 4. The mechanism of DLF may be related to inhibiting lipid peroxide.
出处
《青海医学院学报》
CAS
2004年第1期7-11,共5页
Journal of Qinghai Medical College
