摘要
目的:探讨大蒜素对人胃癌细胞系MGC-803和SGC-7901细胞周期的影响及其与周期特异性药物联合使用,增强抗癌药物对肿瘤细胞的杀伤作用。方法:苔盼蓝拒染法检测细胞增殖抑制率以观察大蒜素对胃癌细胞的增殖抑制作用;流式细胞仪及瑞士-姬姆萨染色光镜观察检测细胞周期的改变;3μg/ml大蒜素与周期特异性抗癌药物博莱霉素A5和长春新碱联合使用,观察药物细胞毒活性的改变。结果:大蒜素可抑制MGC-803细胞生长,24hIC50为6.4μg/ml,也可抑制SGC-7901细胞的生长,24hIC50为7.3μg/ml;以3μg/ml、6μg/ml、9μg/ml终浓度大蒜素分别作用两种胃癌细胞系24h后,与对照组比较,G0/G1细胞周期百分率明显减少,而G2/M期明显增加(P<0.01);6μg/ml大蒜素作用培养细胞24h后,与对照组比较细胞分裂指数明显增加。提示两种细胞系经大蒜素处理后,细胞周期阻滞于M期;大蒜素与两种抗癌药物联合使用,作用于MGC-803细胞,BLMA5和VCR的24hIC50值分别由单独应用的1.210μg/ml和0.125μg/ml下降到0.370μg/ml和0.031μg/ml,作用于SGC-7901细胞,BLMA5和VCR的24hIC50值分别由单独应用的1.760μg/ml和0.287μg/ml下降到0.680μg/ml和0.074μg/ml,显示低剂量大蒜素增强了抗癌药物对肿瘤细胞的杀伤作用。结论:大蒜素可使MGC-803及SGC-7901两种细胞?
Objective:To study the eff ec t of allicin on human gastric cancer cells lines MGC)803and SGC)7901and that com bination of allicin with antitumor drugs enhances cytotoxicity of anti tumor drugs to these cancer cells.Methods:To evaluate the inhibition rates o f proliferation of cells by means of trypan)blue exclusion,to analyze the change of cell cycle using flow cytometry and Giemsa staining,to study the change of c ytotoxicity of BLM A 5 and VCR after combination of allicin3 ì g/ml with these cell cycle phase specific antitumor drugs.Results:Allicin in hibited cell growth of MGC)803,IC 50 of24hours was6.4 ì g/ml;Allicin inhibited cell growth of SGC)7901,IC 50 of24hours was7.3 ì g/ml.These cells were treated by allicin at the concentrations of3 ì g/ml,6 ì g/ml,9 ì g/ml for24hours,compared with the control group,the percentage of G 0/G 1 phase of the cells was decreased and that of G 2/M cells was increased significantly in the allicin treated group(P<0.01).Tr eated by allicin at the concentrations of6 ì g/ml for24hours,compared with the control group,cell division index were muc h higher,it showed that allicin caused arrest of gastricai cancer cells in M pha se.In comparison with IC 50 of BLM A 5 and VCR alone or combined with allicin3 ì g/ml,the24h IC 50 values decreased from1.210 ì g/ml and0.125 ì g/ml to0.370 ì g/ml and0.031 ì g/ml,respectively in MGC)803cells;and decreased from1.760ìg/ml and0.287ìg/ml to0.680ìg/ml and0.074ìg/ml,respec tively in SGC)7901cells.Conclusion:Allicin caused arrest of gastric cancer cells in M phase,and allicin can enhance cytotoxicity of the cell cycle phase speci fic antitumor drugs to these cancer cells.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第4期193-196,共4页
Chinese Journal of Clinical Oncology
基金
国家重大基础研究规划基金项目973资助(编号:G1998051203)
