摘要
采用T3抗体包被酶标板微孔、生物素化T3和辣根过氧化物酶(HRP)标记链亲合素作为信号放大体系,一系列定量游离T3(FT3)为标准品,并以四甲基联苯胺-过氧化氢为显色底物,建立了FT3的生物素-亲合素(BA)酶联免疫分析(FT3-BA-EIA)法。其灵敏度为0.90pmol/L,标准曲线范围0.9～45.0pmol/L,批内变异系数3.1%～8.1%,批间变异系数4.9%～10.6%。测定了101例临床血样,其中正常人46例,实测范围2.5～11.1pmol/L,均值为5.1±2.0pmol/L;27例甲亢病人,实测范围9.7～37.3pmol/L,均值为20.5±7.1pmol/L;20例甲低病人,实测范围0.9～3.8pmol/L,均值为2.0±0.9pmol/L;8例孕妇,实测范围为3.4～8.5pmol/L,均值为6.0±2.5pmol/L。通过对临床血样的检测,本法与化学发光法(CIA)及放免法(RIA)的测定结果有良好的相关性。应用本法可有效地诊断甲状腺疾病,具有操作简便、灵敏、快速的特点,适于临床检测和科研应用。
The methodology for determining FT3 in serum to estimate the patients with thyroidal and related diseases is established. In this new solid-phase antibody enzyme immunoassay, biotinylated T3 and HRP-streptavidin conjugate are used. The detection limit is low (0.90 pmol/L), the measurable range is 0.9 to 45.0 pmol/L, The intra- and inter-assay CVs are 3.1%~8.1% and (4.9)%~10.6%, respectively. FT3 values are obtained with this assay for 101 samples. The FT3 concentrations range is from 2.5~11.1 pmol/L in 46 euthyroid subjects, 9.7~37.3 pmol/L in 27 hyperthyroid patients, and 0.9~3.8 pmol/L in 20 hypothyroid patients and 3.4~8.5 pmol/L in 8 pregnalcy, respectively. Comparing with CIA and RIA, the clinical results correlated well. The method is suitable for clinical and research application.
出处
《同位素》
CAS
2004年第1期39-42,共4页
Journal of Isotopes
