摘要
目的 :本文建立了用高效液相色谱法同时测定双活祛风镇痛酒 (青风藤、丹参等 )中青藤碱和丹参酮ⅡA 的含量的方法。方法 :固定相为WaterμPORASILTM P/N2 74 77柱 ,流动相为甲醇 水 乙二胺 ( 90∶10∶0 .12 5 ) ,检测波长为 2 6 2nm。结果 :青藤碱的平均回收率为 97.9% ,相对标准偏差为 2 .83%。丹参酮ⅡA 的平均回收率为 99.0 % ,相对标准偏差为1.96 %。结论 :该法快速准确 ,重现性好 ,为同类产品提供了质量控制方法。
AIM: A determination was created of sinomenine and tanshinnone Ⅱ A in Shuanghuoqufengzhentong Wine (Caulis Sinomenii, Radix Salviae Miltiorrhizae, etc.) at the same time by HPLC. METHODS: The method contained a Waters μ PORASIL TM P/N27477 column with methanol water ethylene diamine (90∶10∶0.125) as mobile phase, UV detection wavelength was set up at 262nm. RESULTS: The average recovery of sinomenine was 97.9%, the RSD was 2.83%. The average recovery of Tanshinnone Ⅱ A was 99.0%, The RSD was 1.96%. CONCLUSION: The method is rapid, accurate, sensitive. This study provides a testing method for the quality control of similar products.
出处
《中成药》
CAS
CSCD
北大核心
2004年第4期280-282,共3页
Chinese Traditional Patent Medicine
