摘要
目的 研究NF κB在烧伤脓毒症大鼠急性肝损害中的作用。方法 采用 30 %Ⅲ度烫伤加内毒素攻击大鼠模型模拟临床烧伤脓毒症 ,6 3只Wistar大鼠随机分为正常对照组、烧伤脓毒症组、烧伤脓毒症 +NF κB抑制剂二硫代氨基甲酸吡咯烷 (PDTC)组、单纯PDTC处理组 ,采用凝胶电泳迁移率改变分析法(EMSA)分析肝脏NF κB活性 ,免疫印迹 (Westernblotting)法检测肝脏IκB α表达 ,酶联免疫吸附试验检测肝脏TNF α含量的变化。结果 NF κB于伤后 1h明显活化并达到高峰 (P <0 0 5 ) ;IκB α表达先减少 (P <0 0 5 )后逐渐恢复 ;NF κB抑制剂PDTC可以降低NF κB的活性和减少TNF α的释放 ,同时明显降低血清ALT、AST含量。结论 抑制NF κB活性有助于减轻烧伤脓毒症时肝脏的急性损害。
Objective To investigate the potential role of nuclear factor kappa B (NF-κB) in the development of acute liver injury induced by postburn sepsis in rats. Methods Sixty-three Wistar rats were randomly divided into normal control group, burn sepsis group, burn sepsis with pyrrolidine dithiocarbamate (PDTC) treatment group, and simple PDTC treatment group. Rats in experimental groups were subjected to 30% full-thickness scald injury combined with intraperitoneal injection of lipopolysaccharide (LPS) to mimic early sepsis after burns. The following parameters were measured with the method indicated in parentheses: NF-κB activity (electrophoretic mobility shift assay, EMSA), IκB-α expression (Western blotting), TNF-α (enzyme-linked immunoadsordent assay, ELISA). Results NF-κB activity was markedly enhanced and reached its peak at 1 hour after LPS challenge (P<0.05), expression of IκB-α was lowered initially (P<0.05) and then returned to the normal range. After treatment with NF-κB inhibitor PDTC, NF-κB activity and protein expression of TNF-α were decreased, at the same time serum alanine aminotransferase (ALT) and aspartate transferase (AST) levels were also markedly lowered. Conclusion Inhibition of NF-κB activity may attenuate acute liver injury in burn sepsis.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2004年第1期36-38,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金 (编号 30 2 71 339)
全军医学科学技术研究"十五"计划重点项目 (编号 0 1Z0 95)资助课题
