摘要
目的 探讨三碘甲状腺原氨酸 (T3)在神经干细胞 (NSC)分化中的作用以及甲状腺激素受体(TR)mRNA在NSC分化过程中的表达变化。方法 在体外成功诱导扩增NSC ,用T3 对NSC进行诱导分化 ,半定量RT PCR法检测NSC在分化前后TRsmRNA的表达变化 ,免疫细胞化学方法鉴定分化后的细胞类型。结果 T3 可诱导NSC分化为神经元、少突和星形胶质细胞 ,其中髓鞘碱性蛋白 (MBP)阳性细胞约占 80 %。当NSC分化时存在不同的TRsmRNA的时间顺序表达。结论 T3 能诱导NSC向胶质细胞分化 ,是少突胶质细胞分化的重要调控因子 。
Objective To ex pl ore the action of T 3 on neural stem cell (NSC) differentiation and the change of thyroid hormone receptor (TR) mRNA expression during NSC differentiation. Methods Human embryonic brain-derived NSC was suc cessfully expanded into neurospheres with mitogens and identified by immunocytoc hemistry method. T 3 was used to induce the differentiation of NSC. During the NSC differentiation, mRNA expression levels of different TRs were detected by se miquantitative RT-PCR. At the same time the cell types of the differentiated ce lls were identified by immunocytochemistry method. Results After being induced by T 3, NSC could differentiate into neuron, ol igodendrocyte, astrocyte, but myelin basic protein positive cells accounted for 80%. The time sequence expression of different TRs mRNAs existed during NSC diff erentiation. Conclusion T 3 can induce NSC to develop to glial cell and is an important regulator of the oligodendrocyte diffe nrentiation. T 3 exerts distinct effects through time sequence expressions of d ifferent TRs mRNAs.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2003年第5期348-350,共3页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金资助项目 ( 30 2 30 330 )
天津市自然科学基金资助项目 ( 0 1 36 1 1 71 1 )
