摘要
目的 :研究板蓝根组酸活性单体 5b对肝癌耐药细胞BEL 74 0 4 /ADM逆转作用及机制。方法 :利用BEL 74 0 4细胞的多药耐药模型BEL 74 0 4 /ADM ,应用MTT法、免疫组化法和高效液相色谱法(HPLC)观察板蓝根组酸活性单体 5b对BEL 74 0 4 /ADM多药耐药逆转。结果 :板蓝根组酸活性单体 5b能降低肝癌耐药细胞BEL 74 0 4 /ADM对ADM的耐药性 ,细胞MDR ,p gp的表达改变不明显 ,其细胞内ADM的含量明显增高。结论 :板蓝根组酸活性单体 5b能逆转肝癌耐药细胞BEL 74 0 4 /ADM对ADM的耐药性 ,其逆转作用可能与降低P gP药物外排功能、增加细胞内药物浓度有关。
Objective To study the roles and mechanisms of the Isatis tinctoria L conbination acid active single body 5b in reversing the drug resistant of hepatocellular carcinoma cell BEL 7404/ADM.Methods MTT,immunohistochemistry and high performance liquid chromatography (HPLC) were used to detected the reversity of multidrug resistant of Isatis tinctoria L conbination acid active single body 5b in BEL 7404/ADM.Results Isatis tinctoria L conbination acid active single body 5b reduced the drug resistant of hepatocellular carcinoma cell BEL 7404/ADM to ADM.However,the expressions of MDR,p gp were as the same as before,and the concentration of ADM in cell increased greatly.Conclusions Isatis tinctoria L conbination acid active single body 5b could reverse the drug resistant of hepatocellular carcinoma cell BEL 7404/ADM to ADM, which may be attributed to the decreasing of the drug excretion of p gp and the increasing of the concentration of drug in cells.
出处
《肿瘤防治杂志》
2003年第6期580-582,共3页
China Journal of Cancer Prevention and Treatment
基金
广西科技厅资助课题 (桂科攻 99190 40 )
关键词
多药耐药
板蓝根组酸活性单体-5b
免疫组化法
逆转
肝癌细胞
multidrug resistance
Isatis tinctoria L conbination acid active single body 5b
immunohistochemistry
rverse
hepatocellular carcinoma cell
