摘要
用动态光散射法研究了不同浓度NaCl对溶菌酶晶体生长前期溶液中聚集体状态的影响 ,并将这些溶液中的聚集体吸附到硅片表面 ,用原子力显微镜进行了观察 .结果表明 ,在NaCl浓度为 0～ 0 5mol·L-1时 ,随着NaCl浓度的升高 ,溶液中大的聚集体逐渐消失 ,直至基本上只存在几纳米大小的聚集体 .测量了相应条件下溶液的Zeta电势值以说明NaCl与溶菌酶之间的相互作用的变化情况 .本文从溶液中无序聚集体的角度出发提出了判断晶体能否生长的一个可能的标准 ,并对动态光散射与原子力显微镜的结果进行了对比和分析 .
The aggregates in lysozyme solution with different NaCl concentrations in which nuclei have not appeared were studied by dynamic light scattering (DLS) method. In addition, the aggregates absorbed on the silicon wafer surface were observed by atomic force microscope (AFM). The results showed that when the NaCl concentration was lower than 0.4 mol (.) L-1, the higher the NaCl concentration was, the smaller the dimension of the aggregates was. The big aggregates disappeared and only aggregates with the dimension of 3 similar to 4 nm in lysozyme solution remained when NaCl concentration. was 0.4 or 0.5 mol (.) L-1. The result of Zeta potential showed the change of the interaction between NaCl and lysozyme molecules. From the experimental results, we conclude that lysozyme crystal could not grow in solution in the presence of amorphous aggregates bigger than 100 nm in dimension. Additionally, the difference between results of US and AFM was analyzed.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2004年第8期757-761,共5页
Acta Chimica Sinica
基金
国家自然科学基金 (No.30 2 70 30 2 )
中国科学院知识创新工程重要方向 (Nos.KSCX2 SW 32 2
KSCX2 SW L0 5)资助项目
