摘要
目的 :建立体外培养大鼠脑皮层神经元机械性损伤模型 .方法 :SD胎鼠脑皮层神经元体外培养 7d ,微量移液器塑料滴头于培养孔内机械性划割培养之神经元 ,依划割程度不同分为轻、中、重 3组 ,对照组除不进行机械性划割 ,其余处理同损伤组 ,伤后不同时间点 (1 0 ,30min ,1 ,3,6 ,1 2 ,2 4h)检测细胞存活率及培养液上清乳酸脱氢酶 (LDH)含量 .结果 :伤后 30min起 ,损伤轻、中、重组细胞存活率较对照组明显降低 ,且随损伤程度加重 ,细胞存活率下降 (P <0 .0 5 ) ,2 4h达高峰 ,伤后 30min起LDH含量较对照组增加 (P <0 .0 5 ) .结论 :微量移液器塑料滴头机械性划伤操作简便 ,能较好模拟脑损伤后神经元损伤机制 ,造成神经元不同程度损伤 ,利于观察和研究神经元损伤及周围神经细胞反应 。
AIM: To establish a novel mechanically injured model of cortical neuron of rats in vitro . METHODS: The SD fetus (16 17 d) cortical neurons were divided into injury and control groups after 7 days in vitro cultivation. Mechanical injury was caused by plastic tip on the cortical neuronal culture in the injured group, which was subdivided into three groups according to the degree of insults, while the control group underwent all the same managements except for the mechanical injury. Cellular viability and the level of LDH were detected at different times after injury (10, 30 min, 1, 3, 6, 12 and 24 h). RESULTS: Cellular viability of injured groups was obviously lower than that of control group, which was in line with the severity of injury. The lowest level was recorded at 24 h after injury. The concentration of LDH had increased since 30 min after injury. CONCLUSION: Tip caused mechanical injury is easy to perform, which can simulate the pathological mechanism of traumatic brain injury, cause cortical neuronal damage at different levels and is convenient for the study of morphological changes of mechanically injured neurons and the cells around them.
出处
《第四军医大学学报》
北大核心
2004年第4期307-309,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (30 2 70 534)
