摘要
目的 :研究B7 1基因对小鼠前胃癌细胞 (MFC)增殖活性的抑制作用 ,探讨其在肿瘤免疫中的作用。方法 :将B7 1基因转染MFC细胞后 ,用MTT法和流式细胞仪 (FCM )分别测定转染细胞增殖活性的变化 ,并将已转染B7 1的MFC细胞种植到裸鼠皮下 ,研究其对体内成瘤的影响。结果 :转染B7 1基因的MFC细胞增殖活性受到抑制 ,P <0 0 5。结论 :B7 1基因对MFC细胞增殖活性有抑制作用。
OBJECTIVE:To investigate the suppression effect of B7 1 gene on proliferous characteristics of MFC cells.METHODS:MFC was transfected by B7 1 gene with the liposomes induced.The proliferation was assayed by MTT and FCM.Then the MFCs transfected by B7 1 were transplanted into subcutaneous of nude mice.RESULTS:The proliferation of MFCs transfercted by B7 1 was suppressed, P <0 05.CONCLUSIONS:The transfer of B7 1 gene can effectively suppress the proliferation of MFC cells.
出处
《肿瘤防治杂志》
CAS
2004年第2期131-133,共3页
China Journal of Cancer Prevention and Treatment
关键词
胃肿瘤/病理学
基因
转染
鼠科
stomach neoplasms/pathology
gene
transfection
muridae
