摘要
目的 研究肺表面活性物质对分泌性中耳炎的治疗作用 ,为分泌性中耳炎的治疗探索新的途径。方法 采用灭活的肺炎链球菌鼓室内注射 ,建立豚鼠分泌性中耳炎的动物模型 ,鼓室内注射肺表面活性物质 1周后检测听性脑干反应 (auditorybrainstemresponse ,ABR)反应阈的变化 ,观察肺表面活性物质治疗前后咽鼓管黏膜光镜和电镜下的组织形态学变化。结果 豚鼠鼓室内注射灭活肺炎链球菌悬浮液 5d后 :鼓室积液 ,鼓膜浑浊 ,光锥消失 ,ABR平均 ( x±s)反应阈由正常对照组的 (14 0± 3 1)dB提高至 (45 0± 5 7)dB ;豚鼠咽鼓管黏膜层增厚 ,黏膜面有无结构的红染物覆盖 ,纤毛排列紊乱。经肺表面活性物质治疗 1周后 :鼓室积液减少或消失 ,ABR平均反应阈由 (45 0± 5 7)dB降低为(2 3 5± 6 3)dB。t检验分析 ,造模组与肺表面活性物质治疗组反应阈差异有极显著性 (P <0 0 0 1) ;豚鼠咽鼓管黏膜层变薄 ,纤毛排列较整齐 ,朝向鼻咽侧。结论 肺表面活性物质对豚鼠分泌性中耳炎有治疗作用。
Objective To study the effect of pulmonary surfactant on otitis media with effusion in guinea pigs to find a new way to manage otitis media with effusion. Methods Nonviable heat-killed pneumococci (HKP) solution was inoculated into the middle ear cavity in guinea pigs via a transeardrum approach to set up a model of otitis media with effusion in guinea pigs. Seven days after being injected with pulmonary surfactant (PS) by transeardrum approach, ABR threshold and histomorphological changes of eustachian tube mucosa of guinea pigs were examined by light microscopy and scanning microscopy. Results Five days following inoculation of HKB serous effusion were present in the middle ear cavity of guinea pigs, but disappearance of light cone. Response ( ±s ) threshold raised from (14.0±3.1)dB to (45.0±5.7)dB. The eustachian tube mucosa was thickened and lined eosin-stained structureless matter over mucosa, while cilia of eustachian tube mucosa irregularly arranged. Seven days after being treated by PS, serous effusion of tympanum was reduced or disappeared, and response threshold decreased from (45.0±5.7) dB to (23.5±6.3)dB. There was significantly difference between them( P <0.001).Eustachian tube mucosa was thinned, Cilia of eustachian tube mucosa regularly arranged to the nasopharynx. Conclusion Pulmonary surfactant plays a important role in otitis media with effusion of guinea pigs.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2003年第6期445-447,I004,共4页
Chinese Journal of Otorhinolaryngology
基金
上海市高等学校科学技术发展基金资助项目(2 0 0 0C12 )
