摘要
目的 探讨结直肠癌中转化生长因子 β1(TGF β1)的表达与肿瘤浸润转移和血管形成的关系。 方法 免疫组化S P法检测 12 6例结直肠癌中TGF β1和血管内皮生长因子 (VEGF)的表达 ,同时应用CD34标记肿瘤间质中微血管密度 (MVD)。结果 12 6例结直肠癌中TGF β1和VEGF的阳性表达率分别为 4 2 1%和 6 3 5 %。结直肠癌中TGF β1、VEGF蛋白的表达和MVD与肿瘤的浸润深度、淋巴结转移和Dukes分期呈正相关 (P <0 0 5 ) ;VEGF在TGF β1表达阳性的结直肠癌中的阳性表达率高于TGF β1表达阴性的结直肠癌 (P <0 0 5 ) ,TGF β1表达阳性的结直肠癌MVD高于TGF β1表达阴性的结直肠癌(P <0 0 5 )。结论 TGF
Purpose To study the expression of transformating growth factor beta 1 (TGF β1) in colorectal carcinoma and its relationship with invasion, metastasis and angiogenesis. Methods The expression of TGF β1 and VEGF in 126 cases of colorectal carcinoma and microvessel density (MVD) in the neoplasms were detected by S P immunohistochemical staining with monoclonal antibodies against TGF β1, VEGF and CD34. Results The positive rates of TGF β1 and VEGF in 126 cases of correlated carcinoma were 42 1% and 63 5% respectively. TGF β1 and VEGF positive expression and MVD were significantly correlation with the depth of invasion, lymph node metastasis and Dukes staging ( P <0 05), the positive rate of VEGF in colorectal carcinoma with TGF β1 expression was higher than that without TGF β1 expression( P <0 05), and the expression of TGF β1 was closely correlated with MVD ( P <0 05). Conclusion TGF β1 may be involved in invasion and metastasis by directly or indirectly stimulating angiogenesis in colorectal carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
2004年第1期79-82,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
安徽省教育厅科研基金资助(No 2 0 0 1jl2 5 1)
关键词
结直肠癌
TGF-Β1表达
肿瘤
浸润转移
血管形成
colorectal neoplasms
TGF β1
VEGF
microvessel density
angiogenesis
immunohistochemistry
