摘要
目的 检测胶质瘤中Axin基因的点突变及其表达情况 ,初步探讨Axin与胶质瘤发生的关系。方法 采用聚合酶链反应 单链构象多态性 (PCR SSCP)技术及DNA测序方法检测Axin基因外显子 8,9及 10在 2 8例胶质瘤中的突变情况 ;同时对上述胶质瘤及正常脑组织进行免疫组化染色。结果 在 2 8例胶质瘤组织中Axin的第 10个外显子共有 6例样本 (2 1.4 % ) 3处发生了错义突变 ;3例 3处发生了同义突变 ;2 8例胶质瘤中 8例 (2 8.6 % )Axin表达阳性 ,正常脑组织中神经元表达阳性 ,神经胶质细胞表达阴性 ,检测到突变的样本中 1例表达阳性。
Objective To detect the point mutations of Axin gene and its expression in glioma and explore the relationship between Axin gene and the occurrence of human glioma.Methods The point mutations of exon 8,9,10 of Axin gene were analyzed in 28 cases of glioma by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) analysis, silver staining and DNA sequencing. Immunohistochemistry was used to detect the Axin expression in these cases and normal brain tissues.Results Three missense point mutations were detected in exon 10 of Axin gene in 6 cases(21.4% ), in addition,3 silent mutations were detected in 3 cases; Eight cases of glioma(28.6%)showed positive Axin expression, and in the normal brain tissues Axin was expressed in neurons but not in gliocytes. There was one sample expressed Axin among those with point mutations.Conclusion Point mutations of Axin gene may be involved in the occurrence of glioma in human.
出处
《中华神经外科疾病研究杂志》
CAS
2003年第4期334-337,共4页
Chinese Journal of Neurosurgical Disease Research
基金
高等学校骨干教师计划资助项目
归国留学人员科研启动基金资助项目 (1 999747)
