摘要
根据Genbank中猪肺炎支原体P36基因序列设计一对特异性引物,通过聚合酶链式反应(PCR)扩增出猪肺炎支原体ZCF23株特异性蛋白P36基因序列,经测序确认后,克隆入原核表达载体pGEX6p＿1的EcoRI和XhoI位点之间,转化宿主菌BL21,将筛选出的阳性克隆用IPTG诱导,通过SDS＿PAGE电泳进行鉴定,结果表明,P36蛋白基因获得了表达,这为猪肺炎支原体免疫检测与诊断提供了重要条件。
A pair of primers specific for cytosolic P36 gene of Mycoplasma hyopneumoniae were designed,and the P36gene was amplified by polymerase chain reaction(PCR)from Chinese strain ZCF23.The PCR product was recovered and inserted into the inducible expression vector pGEX 6p-1.The recombinant plasmid pGEX6p-1-P36 was transformed into E.coli BL_(21).After induced with IPTG,the fusion protein GST-P36 in BL_(21)(pGEX 6p-1-P36) was revealed successfully by SDS-PAGE.This expresse dprotein would be very useful in the immunological detection and diagnosis of Mycoplasma hyopneumoniae.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2004年第3期188-191,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
受教育部"高校青年教师奖"(175)
江苏省"六大人才高峰"计划(G_2002_026)
江苏省"三药"项目资助
关键词
猪肺炎支原体
P36基因
克隆
表达
Mycoplasma hyopneumoniae
P36 gene
cloning
expression
