摘要
目的用高效液相色谱法测定大枣中芦丁的含量。方法采用PhenomenexLunaC18柱(150×4.6mm,5μm),以甲醇0.05%H3PO4水(4258)为流动相,流速1.0ml/min,检测波长254nm,柱温25℃,用外标法定量,测定大枣中芦丁的含量。结果与结论芦丁的线性范围0.053～0.53μg,相关系数r=0.9999,回收率99.22%,RSD2.86%,该方法简便,快速,线性关系良好。
Objective: To determine the content of Rutin in Ziziphus jujuba. Methods: The HPLC method was used to determinethe content of Rutin in Ziziphus jujuba. The analysis was carried on Phenomenex Luna C18 column. The mobile phase was MeOH-0.05% H3PO4(42:58), flow-rate 1.0ml/min, wave-length 254nm and temperature 25℃. Results and conclusion: The method wassimple and had good linear relationship.The linear range was 0.053～0.53μg and gave a correlationship of 0.9999. The recoverywas 99.22% and RSD 2.86%. The method was easy in to determineing the content of Rutin in Ziziphus jujuba.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2004年第4期147-149,共3页
Food Science
