摘要
目的 :横纹肌肉瘤是儿童期最常见的软组织肉瘤 ,因其组织学常呈现低分化状态 ,故与其它儿童小圆细胞瘤的鉴别诊断十分困难。本实验初步探讨应用Westernblotting方法检测生肌转录因子基因家族成员之一—Myogenin蛋白以作辅助诊断的可行性。方法 :提取 8例横纹肌肉瘤及 2 0例其它儿童实体肿瘤新鲜组织中的蛋白质 ,进行SDS—PAGE电泳 ,然后将蛋白转移至硝酸纤维膜 ,作Westernblotting蛋白质杂交 ,对印迹进行分析。结果 :8例横纹肌肉瘤均有明显的Myogenin蛋白印迹 ,在 1例肾母细胞瘤中呈假阳性 ;用Westernblotting方法可检测到仅 5 μg组织蛋白中Myogenin蛋白的表达。 结论 :用Westernblotting方法检测Myogenin蛋白对于横纹肌肉瘤具有极高的灵敏度和较好的特异性 ,因而有一定诊断应用价值。
Objective:Rhabdomyosarcoma (RMS), the most frequent soft tissue tumor, accounts for about 5～8% of all pediatric malignant solid tumor. Histologic diagnosis of RMS in the poorly differentiated cases is very difficult. Thus the pathologists are often faced with the challenge of accurately differentiating RMS from other small round cell tumors. Our experiment attempt to detect Myogenin protein in RMS with Western blotting and try to use it for differential diagnosis.Methods:Extracting protein from 8 cases of RMS and 20 cases of other childhood tumors. The protein were resolved through the SDS-PAGE gel, transferred to a membrane and tested for Western blotting with the antimyogenin antibody.Results:Myogenin protein were detected in all 8 cases of RMS and a Wilms' tumor showed false-positive. We observed the expression of Myogenin protein from only 5 μg tumor tissue protein.Conclusion:Western blotting is a very sensitive and specific method for detecting Myogenin protein in RMS and useful for diagnosis of RMS.
出处
《临床肿瘤学杂志》
CAS
2004年第2期126-127,130,共3页
Chinese Clinical Oncology
