摘要
背景:神经细胞内钙超载引起一系列病理生化反应,从而对神经细胞造成损害。应用Ca2+通道阻滞剂药物治疗颅脑外伤为此提供了理论依据。目的:探讨降低Ca2+-PO43-在电感耦合等离子体原子发射光谱(ICP-AES)法中的干扰,制定合适的分析条件,测定大鼠脑挫伤后脑组织中钙含量的变化。设计:随机对照实验。地点和材料:实验地点:蚌埠医学院检验系医学化学实验室。选择体质量200～300g雄性wistar大鼠18只(由本院动物科提供),随机分为Ca2+对照组及外伤组,每组9只。干预:实验不同浓度P(V)(0～2000mg/L)在5个观测高度,对Ca2+谱线强度的影响;阳极电流改变时2000mg/LP(V)对Ca2+的影响;载气压力改变时P(V)对Ca2+的影响。用ICP-AES法检测脑挫伤后脑细胞内游离钙的浓度,并作病理学观测。主要观察指标:脑挫伤后脑不同部位组织内的钙含量变化。结果:本实验制定的分析条件可以明显降低PO43-的干扰,20次测定的相对标准偏差(RSD%)为1.16,样品加标回收率为97%。脑挫伤后脑细胞内游离钙明显增加。脑挫伤后,各脑区细胞内钙含量都明显高于对照组(t=3.45～46.39,P<0.01)。结论:脑挫伤可能引起脑神经细胞膜上钙通道开放、钙离子大量内流,引起钙超载。优化ICP-AES实验条件对降低干扰提高分析结果的准确度至关重要。
BACKGROUND:Intracellular calcium overload in neurocyte induces serials of path ophysiological reaction and therefore,causes injuries in neurocyte.The applicati on of Ca2+channel blocking agent in the treatment of craniocerebral trauma prov ides a theoretical gist. OBJECTIVE:To discuss the interference of Ca2+-PO43-in the measure of induct ance coupling plasma atom emission spectrum(ICP-AES) and to set proper analytic al conditions for the assay of the changes of cerebral calcium content after cer ebral contusion in rat. DESIGN:A randomized controlled study. SETTING and MATERIALS:The experiment was completed in Medical Chemical Laborat ory of Analysis Department of Bengbu Medical College.Eighteen male Wistar rats w ith a body weight from 200 g to 300 g obtained from animal department of our Uni versity were randomly allocated into Ca2+control group and trauma group with 9 rats each. INTERVENTION:To study the impacts of different strength P(V)(0-2 000 mg/L) at 5 observation heights on the intensity of Ca2+spectrum;the impact of 2 000 mg/ L of P(V) on Ca2+when the current at positive pole changed;and the impact of P( V) on Ca2+when air pressure changed.Cerebral intracellular free calcium strengt h after cerebral contusion was assayed by ICP-AES and the pathological changes were observed as well. MAIN OUTCOME MEASURES:The content changes of calcium at different cerebral are as after cerebral contusion. RESULTS:The analytical condition set in our study could significantly decrease the interference of PO43-,with the relative standard deviation(RSD%) of 1.16 for 20 times of assays and 97%of rate of recovery in marked samples.Cerebral in tracellular free calcium significantly increased after cerebral contusion.Intrac ellular calcium contents at each cerebral area were significantly higher than th at of control group(t=3.45-46.39,P< 0.01). CONCLUSION:Cerebral contusion could lead to the openness of calcium channel on the membrane of cerebral neurocyte, which would cause great intra-flow of calc ium ions to induce calcium overload.It is extremely important to optimize the ex perimental conditions of ICP-AES for the decrease of the interference and the i ncrease of the analytical accuracy.
出处
《中国临床康复》
CSCD
2004年第13期2581-2583,共3页
Chinese Journal of Clinical Rehabilitation
基金
蚌埠医学院科研基金资助项目(1995-017)~~
