摘要
以龙牙百合鳞片叶切块为外植体 ,通过多种培养基的比较试验 ,得出MS +2 ,4 -D 2mg/L +6 -BA0 2mg/L是诱导愈伤的最佳培养基 ,MS +6 -BA 1mg/L +NAA 0 0 5mg/L是不定芽诱导及伸长的适宜培养基。MS +NAA 2mg/L是生根的最佳培养基。本试验已建立了适用于龙牙百合遗传转化的快速高频再生系统。用携带有几丁质酶基因和 β - 1、 3葡聚糖酶基因的工程菌 ,通过农杆菌介导法和基因枪转化法转化龙牙百合 ,经PCR和点杂交检测证明外源基因已经整合到植物染色体中。同时对农杆菌介导法和基因枪法进行比较 ,发现农杆菌介导法的转化率为 16 7% ,基因枪法的转化率为 5 0 % ,因此可能基因枪转化法更适于龙牙百合的遗传转化。
The scale leaf of Longya Lilium( lilium spp )was taken as explant Through the comparism experiment of different media,we got the result:the combination of MS +2,4-D 2mg/L+6-BA 0 2mg/L was the optimum medium for the induction of calli;the combination of MS +6-BA 1mg/L +NAA0 05mg/L was the best medium for shoot differentiation and elongation;the medium of MS +NAA2mg/L was the best for adventitious root The engineering bacterium which carried both I-Chi and I-Glu cDNA was pCG-II Two methods of Agrobacterium mediated and gene gun were used to transformate Longya Lilium The results of PCR analysis and southern dot blotting hybridization demonstrated that the Chi and Glu cDNA has been intergrated into host genome At the same time,compared Agrobacterium mediated method with gene gun method,the transformation frequency of the former was 16 7%,while the latter was 50%,so gene gun transformation method was suitable for Longya Lilium
出处
《分子植物育种》
CAS
CSCD
2003年第4期465-474,共10页
Molecular Plant Breeding
