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串联亲和纯化(TAP)技术在蛋白质组学中的应用 被引量:5

Tandem Affinity Purification Technique and Its Application in Proteomics
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摘要 蛋白质是各种生命活动的主要执行者 ,因此构建蛋白质相互作用的网络图对于准确理解蛋白质功能、揭开各种细胞活动的奥秘十分重要 .串联亲和纯化 (TAP) ,是近年来发展出来的一种能够快速研究在生理条件下蛋白质相互作用 ,揭示蛋白质复合体相互作用网络的新技术 ,已成为研究蛋白质组学的一个重要工具 .随着该技术的不断完善 ,TAP技术在认识蛋白质相互作用的过程中必将发挥越来越重要的作用 . Proteins are core executors of biological activity inside cells, therefore, construction of protein-protein interaction network is so important to interpret protein function and decipher the secret of various cellular phenomena. Tandem affinity purification ( TAP) is recent advance in technology made over past few years now enables the study of protein-protein interaction in vivo and the knowledge gathered from such methods will decipher the protein complexes network. Now, it is also a powerful tool available for proteomics research. This new technology will continue to be developed as it is now became extraordinary valuable to study protein-protein interaction.
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2004年第4期379-383,共5页 Progress In Biochemistry and Biophysics
基金 国家自然科学基金资助项目 ( 3 0 170 0 80 3 0 2 70 75 3 ) 国家高技术"863"计划 ( 2 0 0 1AA2 2 2 0 5 3 2 0 0 2AA2 12 0 5 2 0 0 2AA2 0 70 0 6) 国家重点基础研究发展规划项目 ( 973 ) ( 2 0 0 2CB1113 0 1)~~
关键词 串联亲和纯化 TAP 蛋白质组学 网络图 免疫共沉淀 纯化 TAP (tandem affinity purification) proteomics protein-protein interaction network
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