摘要
用扩增片段长度多态性 (AFLP)方法对木荷、锥栗、厚壳桂在广东省鼎湖山 3个不同群落 :针叶林群落 ,针阔叶混交林群落 ,常绿阔叶林群落中的遗传多样性进行了研究。其中木荷和锥栗样品分别在上述 3个群落中采集到 ,总样品数都为 4 8个 ;厚壳桂样品只在针阔叶混交林群落和常绿阔叶林群落中采集到 ,总样品数为 4 0个。研究中把各物种同一群落中的所有个体视为一亚种群。木荷的AFLP分析结果表明 ,4组引物对分别扩增出 2 4、2 7、4 0和 2 7条带 ,其中分别有 1 5、2 3、2 3和 1 6条是多态性带 ,其 3个亚种群的平均遗传多样性分别是 0 35 3、0 336、0 30 4 ;锥栗的AFLP分析结果为 4组引物对分别扩增出 2 7、2 0、33和 39条带 ,其中分别有 1 5、1 5、1 8和 2 6条是多态性带 ,其3个亚种群的平均遗传多样性分别是 0 1 97、0 2 97、0 31 1 ;厚壳桂的AFLP分析结果为 4组引物对分别扩增出 2 3、30、4 2和 31条带 ,其中分别有 1 2、1 9、37和 1 8条是多态性带 ,其 2个亚种群的平均遗传多样性分别是 0 2 85、0 2 95。上述结果的产生既是这 3个物种的生物学特性的反映 ,也是不同群落微环境不同的反映。
Amplified fragment length polymorphism(AFLP)was used to analyze three species: Schima superba,Castanopsis chinensis,Cryptocarya chinensis located across three different communities:coniferous community,mixed coniferous and broad\|leaf community,evergreen broad\|leaved community in Dinghu Mountain(112°31′39″E,23°09′21″N),China.These three communities represent three successional stages.Samples of Schima superba and Castanopsis chinensis were collected in these three communities,while samples of Cryptocarya chinensis were collected in later two communities.Individuals of each species belonging to the same community were considered as subpopulation.Four AFLP primer combinations(EcoR1,EcoR2,Mse1,Mse2)were used in total 48 individuals of Schima superba amplified 24,27,40 and 27 reliable bands,of which 15,23,23 and 16 were polymorphic respectively.The mean population genetic diversities of three subpopulations of Schima superba were 0 353,0 336 and 0 304.Similarly,total 48 individuals of Castanopsis chinensis amplified 27,20,33 and 39 reliable bands,of which 15,15,18 and 26 were polymorphic respectively.The mean population genetic diversities of three subpopulations of Castanopsis chinensis were 0 197,0 297 and 0 311.Total 40 individuals of Cryptocarya chinensis amplified 23,30,42 and 31 reliable bands,of which 12,19,37 and 18 were polymorphic respectively.The mean genetic diversities of two subpopulations of Cryptocarya chinensis were 0 285 and 0 295.These results were caused by both species biological characteristics and microenvironments of different communities.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2004年第2期32-37,共6页
Scientia Silvae Sinicae
基金
广东省自然科学基金资助项目 ( 0 3 12 64 )
原中国科学院华南植物所所长基金资助项目
关键词
鼎湖山
群落演替
遗传多样性
木荷
锥栗
厚壳桂
AFLP
Schima superba, Castanopsis chinensis, Cryptocarya chinensis, AFLP, Genetic diversity
