摘要
用高效液相色谱法测定鸡肝组织中常山酮的残留量。色谱柱为KromasilC18(250mm×4.6mm,5μm);流动相为乙腈-醋酸盐缓冲液(0.25mol/L,pH4.3)-水(5∶3∶12),检测波长243nm,流速2.0mL/min。该方法线性范围为0.10～5.00mg/mL,r=0.9999,平均回收率达到77.0%～80.3%,RSD<4.3%。该方法灵敏、准确、重现性好。
A method of high performance liquid chromatography for halofuginone hydrobromide residues in chiken liver was established with a C_(18) column of 250 mm×4.6 mm(i.d.) and acetonitrile-acetate buffer (0.25 mol/L, pH=4.3)-water (5∶ 3∶ 12,V/V/V) as a mobile phase. The flow rate was 2.0 mL/min. Halofuginone residues were detected at 243 nm with UV detector.The average recoveries ranged from 77.0% to 82.3%with RSD<(4.3%.)
出处
《中国兽药杂志》
2004年第4期11-13,共3页
Chinese Journal of Veterinary Drug
