摘要
探讨脑梗死患者血管紧张素转化酶基因多态性与血浆血管紧张素Ⅱ水平的关系。应用聚合酶链反应测定 173例高血压脑梗死患者血管紧张素转化酶基因插入 /缺失 (D/I)多态性以及用酶联免疫吸附测定法测定血浆血管紧张素Ⅱ水平 ,并与正常对照组比较。结果发现 ,脑梗死组血管紧张素转化酶DD基因型频率为 0 .39,明显高于对照组的 0 .2 4 (P <0 .0 1)。进一步分析发现这种异常与发病年龄≤ 6 0岁组血管紧张素转化酶DD基因型频率明显增高有关。脑梗死组中血浆血管紧张素Ⅱ水平为 2 9.8± 10 .2ng/L ,与对照相比差异无显著性意义 (P >0 .0 5 ) ,但血管紧张素转化酶DD基因型者血浆血管紧张素Ⅱ水平显著高于对照组和同组DI基因型和Ⅱ基因型者 (P <0 .0 1)。发病年龄≤ 6 0岁血管紧张素转化酶DD基因型者血浆血管紧张素Ⅱ水平增高最明显。结果提示 ,血管紧张素转化酶DD基因型是脑梗死发病危险因素 ,发病年龄小于 6 0岁的脑梗死者可能与血管紧张素转化酶D等位基因频率增高、血浆血管紧张素Ⅱ水平增高有关。
Aim To study the relationship between angiotensin-converting enzyme (ACE)gene polymorphism and level of angiotensin Ⅱ (AngⅡ) in plasma with cerebral infarct (CI) in Chinese Han nationality. Methods 173 patients with cerebral infarct and 116 control subjects in Chinese Han were examined using polymorase chain reaction (PCR) and genetic methods. Plasma AngⅡ level determined by enzyme-linked immunosorbent assay (ELISA) method. Results ACE DD genotype appeared more common in patients of cerebral infarct compared with normal controls (0.39 versus 0.24, P<0.01), and it is important in frequency of ACE DD genotype abnormal increase in group of cerebral infarct onset age within 60 years. There were no significantly different in plasma AngⅡ level between cerebral infarct and control subjects, but cerebral infarct with ACE DD genotype group higher plasma AngⅡ level significantly higher than control group and cerebral infarct with ACE DI and II genotype (P<0.01), the level of plasma AngⅡ were increased most remarkably in group of cerebral infarct onset age within 60 years. Conclusions The ACE gene deletion polymorphism might serve as a risk factor for cerebral infarct in Chinese Han nationality while the level of plasma AngⅡ increased might related to group of cerebral infarct onset age within 60 years. Relate to gene deletion polymorphism.
出处
《中国动脉硬化杂志》
CAS
CSCD
2004年第1期54-56,共3页
Chinese Journal of Arteriosclerosis
基金
温州市科委资助项目 (S990 3 0 2D)
