摘要
目的探索根据体重变化监测肝硬化诱导过程中大鼠对硫代乙酰胺 (thioacetamide,TAA)反应的个体差异 ,以提高肝硬化形成率和质量。方法雄性Wistar大鼠 4 6只 (2 0 0～ 2 30 g) ,随机分为 3组。 1组 (2 0只 )以 0 0 3% (w/v)TAA作为饮用水 ,共 12周。 2组 (2 0只 )以 0 0 3%TAA作为初始浓度 ,然后根据每周体重变化调整饮用水中TAA浓度。 3组 (6只 )给予饮用水 ,作为对照组。结果 1组大鼠病死率为 30 % (6 / 2 0 ) ,肝硬化形成率仅 4 5 % (9/ 2 0 )。 2组病死率为 0 ,肝硬化形成率为 90 % (18/ 2 0 )。结论肝硬化诱导过程中大鼠对TAA反应的个体差异可根据每周体重变化加以监测。该法可降低大鼠病死率至 0 ,同时显著提高肝硬化形成率和质量。
Objective To develop a reliable and reproducible technique in which rat′s hepatic and systemic responses to thioacetamide (TAA) during induction of cirrhosis were monitored by weekly weight changes. Methods Male Wistar rats (200～230 g) were divided into three groups. Group 1 (n=20) received continuous administration of 0.03% (w/v) TAA in the drinking water for 12 weeks. Group 2 (n=20) received the same concentration of 0.03% TAA as an initial concentration that was modified according to weekly weight changes in response to TAA during the induction of cirrhosis. Group 3 (n=6) served as controls. Results The mortality in Group 1 was 30% (6/20) and cirrhosis developed in only 45% (9/20). In contrast, there were no deaths in Group 2 and macronodular cirrhosis was found in 90% (18/20) of the rats. ConclusionsVariations in response to TAA can be easily monitored by weekly weight changes to reduce mortality to zero and simultaneously increase the production and quality of cirrhosis induced in the rat.
出处
《中华普通外科杂志》
CSCD
北大核心
2004年第4期239-241,共3页
Chinese Journal of General Surgery
基金
江苏省卫生厅重大课题资助项目 (H2 0 0 2 0 4)
