摘要
目的 观察氰戊菊酯对卵巢颗粒细胞钙稳态、卵巢组织钙稳态相关酶及血清类固醇激素水平的影响。方法 用激光共聚焦显微镜、放射免疫法和定磷比色法、酶免疫法等技术测定氰戊菊酯对人卵巢颗粒黄体细胞钙稳态和 30d染毒大鼠卵巢组织Ca2 + ATP酶、磷酸化酶a(P a)活性、钙调素含量和血清雌二醇、孕酮水平。结果 氰戊菊酯浓度为 2 0 0和 2 0 μmol/L时 ,可见人卵巢颗粒黄体细胞内游离Ca2 + 浓度缓慢上升 ,外钙内流为胞内钙升高的主要来源。染毒大鼠Ca2 + ATP酶活性总体趋势下降 ,对照组为 (10 3 9± 10 5 7) μmol磷·g蛋白 -1·h-1,1/ 2 5 0LD50 为 (80 6± 5 11) μmol磷·g蛋白 -1·h-1;P a活性增高 ,均显著高于对照组 ;钙调素含量升高 ,动情期显著升高 ,对照组为 (35 7± 2 32 ) μg/L ,1/ 2 5 0LD50 为 (90 5± 30 8) μg/L ,1/ 15LD50 为 (110 6± 36 7) μg/L ;血清雌二醇水平上升 ,对照组为 (0 2 6± 0 13)nmol/L ,1/ 2 5 0LD50 为 (0 4 1± 0 19)nmol/L ;孕酮水平下降 ,对照组为 (7 0 5± 5 6 7)nmol/L ,1/ 15LD50 为 (3 35± 1 14 )nmol/L。结论 氰戊菊酯干扰卵巢颗粒细胞内钙稳态 ,影响体内激素水平 ,钙信号通路可能为其影响机制之一。
Objective To observe the effects of fenvalerate(Fen) on ovarian calcium homeostasis. Methods hGLCs were obtained from pre-ovulatory follicles in an in vitro fertilization program,and were cultured for 72 hours. Changes in cellular [Ca 2+ ]i induced by Fen in hGLCs were detected with laser scanning confocal microscopy(LSCM) by using the fluorescent Ca 2+ indicator fluo-3/AM. SD female rats were divided into four groups(control,1/15LD_ 50 , 1/50 LD_ 50 and 1/250 LD_ 50 )in experiment. The activitity of ovarian Ca 2+ -ATPase and phosphorylase A (P-a) and the contents of calmodulin(CaM)were assessed after a 30-day Fen exposure. In addition, serum estradiol-17β(E_2) and progestrone(P_0) concentration were measured by radioimmunoassay, which the sampling rats were ensured at diestrus stage before killed according to vaginal smear.Results 20.0 and 2.0 μmol/L Fen induced the increased of [Ca 2+ ]i in hGLC. This [Ca 2+ ]i increase mostly resulted from Ca 2+ influx in the studied concentration. Fen had shown the inhibition effects on activity of Ca 2+ -ATPase in 1/250 LD_ 50 group( P <0.001) while the activity of phosphorylase A(P-a) in treated groups had significantly enhanced than those of in control . The contents of CaM in ovaries were found to be increased in treated groups. E_2 in 1/250 LD_ 50 group were higher while P_0 in 1/15 LD_ 50 group were significantly lower( P <0.05). Conclusion Exposure to Fen interferes the serum steroid hormone concentrations partly through calcium signal pathway.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2004年第1期18-21,F004,共5页
Chinese Journal of Preventive Medicine
基金
基础研究重大项目前期研究专项项目 (国科基字[2 0 0 1] 5 0号 )
国家自然科学基金资助项目 (3 0 2 7113 4)
