摘要
目的 探讨应用生物可降解材料:聚羟基丁酸/聚羟基戊酸共聚物(Poly-hydroxybutyrate/hydroxyvalerate,PHBV),聚乙醇酸/聚乳酸共聚物(Polyglycolic acid and Polylactic acid,PLGA)构建组织工程心脏瓣膜的可行性。方法 应用扫描电镜观察两种材料的结构特点。将两种材料进行兔皮下包埋,分别于包埋后2、4、6、8、10、12周观察材料的生物相容性和降解率。体外培养犬主动脉瓣问质细胞,研究其生长曲线,平滑肌α肌动蛋白表达及透射电镜扫描下的超微结构。将体外培养的犬主动脉瓣间质细胞分别种植于两种材料上,观察其生长情况并测定细胞合成前列环素的功能。结果 扫描电镜下两种材料均呈网孔状泡沫结构,PHBV孔径约130μm,PLGA孔径约170 μm。皮下包埋实验显示两种材料生物相容性好,PHBV体内降解时间约10周,PLGA体内降解时问约12周。犬主动脉瓣间质细胞平滑肌α肌动蛋白部分阳性表达,细胞内有大量粗面内质网,细胞培养生长曲线良好。种植实验显示细胞在两种材料上均生长良好,并有合成、分泌前列环素的功能(P<0.05)。结论PHBV、PLGA两种生物可降解材料均适合于组织工程心脏瓣膜支架材料的应用。
Objective To evaluate the feasibility to fabricate tissue engineered heart valve leaflet with polyhydroxybutyrate/hydroxyvaler-ate(PHBV) and polylactic/glycolic acid(PLCA)scaffold.Method Constructive characteristics of PHBV and PLGA were observed by scanning electron microscope.PHBV and PLGA were implanted subcutaneously in rabbit and removed at 2,4,6,8,10,12 weeks to study its biocompati-bility and biodegradability. Cell growth curve, smooth muscle-α actin and ultra-microscopic characteristics were studied in canine aortic valve interstitial cells.The cells were seeded on the PHBV and PLGA,and their,function of PGI2 synthesis was determinated.Results The pore sizes of PHBV, and PLGA were 130μm and 170 μm,repectivery.The two materials had good biocompatibility and were completely degraded at the end of 10th or 12 th week. Canine aortic valve interstitial cells expressed smooth muscle-a actin and grew well. Cells implanted on PHBV and PLGA were grew well, and they could synthesize and secrete PGI2 ( P < 0.05) Conclusion PHBV and PLGA can be used to fabricate a three-dimensional, biodegradable tissue engineering heart valve scaffold.
出处
《武警医学》
CAS
2004年第4期269-273,共5页
Medical Journal of the Chinese People's Armed Police Force
基金
国家自然科学基金资助项目(No.39970733)
关键词
生物可降解聚合物
组织工程
心脏瓣膜
实验
生物材料
Polyhydroxybutyrate/hydroxyvalerate(PHBV) Polyglycolic acid polylactic acid(PLGA) Tissue engineering Heart valve
