摘要
目的:探讨在高浓度胰岛素条件下,体外培养的兔视网膜Müller细胞的血管内皮细胞生长因子(vas-cularendothelialgrowthfactor,VEGF)的变化。方法:在不同浓度胰岛素条件下(4,8,12kU/L),体外培养兔视网膜Müller细胞,采用免疫细胞化学法、原位杂交法,定性测定Müller细胞分泌VEGF的变化,采用ELISA方法,定量测定Müller细胞分泌VEGF的变化。结果:高浓度胰岛素能明显增强VEGF的表达(P< 0.05)。结论:高浓度胰岛素可能通过刺激Müller细胞编码VEGF基因的转录,进而增强VEGF蛋白的表达,而在糖尿病视网膜病变的新生血管生成中发挥重要作用。
AIM: To study the effect of high concentration insulin on the expression of vascular endothelial growth factor (VEGF) in cultured rabbit retinal Müller cells in vitro .· METHODS: Müller cells were cultured with insulin of different concentrations (4×103 , 8×103 , 12×103 U/L). Immunocytochemistry,in situ hybridization and ELISA were conducted to assay the expression of VEGF in cultured Müller cells in vitro at different insulin concentrations qualitatively and quantitatively.· RESULTS: VEGF expression was enhanced obviously by high concentration of insulin.· CONCLUSION: Insulin plays an important role in neovascularization of diabetic retinopathy by stimulating the transcription of the VEGF gene in Müller cells so as to enhance the expression of VEGF protein.·
出处
《国际眼科杂志》
CAS
2004年第2期201-205,共5页
International Eye Science
基金
中国吉林省科学基金资助项目(No.990575-5)~~
