摘要
目的评价酶联免疫吸附试验(ELISA)用于天疱疮血清学诊断的意义。方法用ELISA法检测49例天疱疮患者和36例非天疱疮对照者血清,同时以间接免疫荧光法(IIF)检测患者血清抗桥粒芯糖蛋白(Dsg)抗体滴度,比较两者的检测结果。结果27例寻常型天疱疮(PV)、14例落叶型天疱疮(PF)和6例非天疱疮大疱性疾病患者Dsg1ELISA呈阳性,27例PV、1例PF及1例非天疱疮大疱性疾病患者Dsg3ELISA呈阳性。Dsg1ELISA的敏感性和特异性分别是81.25%和88.89%,Dsg3ELISA的敏感性和特异性分别是81.82%和97.22%。与IIF相比,两者检测结果差异有显著性(χ2=4.31,P<0.05)。结论ELISA是一种简便、敏感和特异性高的天疱疮血清学诊断方法,并能辅助鉴别PV和PF。
Objective To evaluate the significance of serum anti-desmoglein (anti-Dsg) autoantibodies in the sero-diagnosis of pemphigus. Methods Serum anti-desmoglein autoantibody levels were measured by ELISA and indirect immunofluorescent assay (IIF) in 49 patients with pemphigus, and in 36 non-pemphigus controls. Results Dsg1 antibody was positive by ELISA in 27 of 33 patients with pemphigus vulgaris (PV), 14 of 16 pemphigus foliaceus (PF) and 6 of 36 controls, and Dsg3 antibody was positive by ELISA in 27 of 33 PV, 1 of 16 PF and 1 of 36 controls. The sensitivity and specificity of Dsg1 ELISA were 81.25% and 88.89%, while those of Dsg3 ELISA were 81.82% and 97.22%, respectively. There was a significant difference of positivity rates between ELISA and IIF (χ2 = 4.31, P < 0.05). Conclusions Detection of serum anti-desmoglein autoantibodies by ELISA provides a simple, sensitive and specific assay for the sero-diagnosis of pemphigus, which might be useful for differentiation of PV and PF.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2004年第2期83-85,共3页
Chinese Journal of Dermatology
关键词
酶联免疫吸附试验
检测
血清
天疱疮
抗体
诊断
Enzyme-linked immunosorbent assay
Pemphigus
Fluorescent antibody technique, indirect
