摘要
目的探讨人乳头瘤病毒(HPV)感染与外生殖器部位表皮增生性疾病发生的相关性。方法采用通用型引物PCR方法对151例外生殖器表皮增殖性病变进行HPVDNA的检测,应用限制性内切酶片段长度多态性(RFLP)方法进行HPVDNA分型。结果30例尖锐湿疣HPVDNA全部阳性,其中HPV6占60%,HPV11占26.7%;40例Bowen样丘疹病、5例生殖器部位Bowen病、6例Queyrat增生性红斑HPVDNA的阳性率分别为55%、100%、33.3%,主要型别为HPV16;18例外生殖器浸润性鳞状细胞癌中HPVDNA的阳性率为27.8%,均为HPV16;32例黏膜白斑和20例乳房外Paget病HPVDNA均为阴性。结论尖锐湿疣主要由HPV6,其次由HPV11感染所致;Bowen样丘疹病、表皮原位癌包括Bowen病、Queyrat增生性红斑的发生可能与HPV16感染有密切关系;外生殖器浸润性鳞状细胞癌中,HPV16感染可能是多种致癌因素中的一个重要因素;外生殖器部位黏膜白斑和乳房外Paget病的发病可能与HPV感染无关。
Objective To study the possible relationship between human papillomaviruse (HPV) infection and external genital proliferative diseases. Methods HPV DNA was detected using polymerase chain reaction (PCR) with a consensus primer and typed by using restriction fragment length polymorphism (RFLP) method in 151 paraffin-embedded biopsy specimens from seven kinds of external genital epidermal proliferative lesions. Results In 30 cases of condyloma acuminatum, HPV DNA was positive for all cases, among which HPV6 and HPV11 accounted for 60% and 26.7%, respectively. In 40 cases of bowenoid papulosis, 5 cases of genital Bowen′s disease and 6 cases of erythroplasia of Queyrat, the positive rates of HPV DNA were 55%, 100% and 33.3%, respectively, and the predominant type was HPV16. In 18 cases of external genital invasive squamous cell carcinoma HPV DNA positive rate was 27.8%, and HPV16 was detected in all of the 5 HPV-positive cases. HPV DNA was negative in 32 cases of leukoplakia and 20 cases of extramammary Paget′s disease. Conclusions Condyloma acuminatum is mainly caused by HPV6 infection, followed by HPV11; HPV16 infection is closely associated with the pathogenesis of bowenoid papulosis and external genital squmous cell carcinoma including Bowen′s disease and erythroplasia of Queyrat. In external genital invasive squamous cell carcinoma, HPV16 infection may be one of the multiple carcinogenic factors. No clear relationship is found between HPV infection and leukoplakia or extramammary Paget′s disease.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2004年第5期280-282,共3页
Chinese Journal of Dermatology
关键词
外生殖器表皮肿瘤
HPV
DNA
检测
分型
人乳头瘤病毒感染
Papillomavirus, human
Condylomata acuminata
Carcinoma, squamous cell
Precancerous conditions
Paget′s disease, extramamary
