摘要
目的研究基质金属蛋白酶-3 (matrix metalloproteinase-3,MMP-3)在类风湿关节炎(RA)患者血清中的水平及其相关影响因素,探讨MMP-3在类风湿关节炎发病中的作用机制,比较在RA不同时期,血清MMP-3的变化,为临床治疗、预后判断提供新的理论依据。方法酶联免疫吸附实验(ELISA)测定47例初诊RA患者和31名正常健康对照血清中MMP-3水平。同时测定其治疗前的其他实验室指标:类风湿因子(RF)、抗角蛋白抗体(AKA)、抗核周因子(APF)、血沉(ESR)及手X片。分析各指标之间的关系。结果47例RA患者MMP-3血清水平为(47.3±1.8) ng/ml,对照组为(12.0±1.3) ng/ml,两组比较差异有显著性(P<0.01)。而无论病例组或对照组,其MMP-3水平差异无显著性。MMP-3水平和RA患者的受累关节数目(P<0.05)、X线分期严重程度及ESR (P<0.01)呈正相关;而与年龄、病程长短、晨僵时间、功能分级、RF、AKA、APF、抗CCP抗体水平无明显关系。不同X线分期时,其MMP-3水平确有差别,其中Ⅰ期与Ⅱ期,Ⅰ期与Ⅲ期差异有显著性(P<0.05)。结论MMP-3在RA血清中高水平存在,与性别无关。尽管RA存在显著的个体差异,MMP-3仍可作为除ESR、RF外的反映病情活动的指标。X线Ⅰ期与Ⅱ期时,MMP-3增高最明显。提示血清中出现高水平MMP-3时,应警惕已有关节软骨的破坏。
Objective To determine serum matrix metalloproteinase-3 (MMP-3) level in patients with rheumatoid arthritis and the factors that may affect it in order to provide new method for diagnosis and therapy.Meth-ods MMP-3 serum level was tested by ELISA in 47 patients with early RA.The serum levels of AKA?APF?ESR?RF?anti-CCP and hand X-ray were measured at the same time.Results Serum level of MMP-3 was obvious higher than normal matched control (P<0.01),but there was no difference in men and women.MMP-3 level was positively cor-related with the number of the damaged joint,X-ray stage and ESR level;but had no obvious relation with age,the dis-ease course,length of morning stiffness,stage of function,and RF,AKA,APF,anti- CCP levels.In different stage of X-ray presentation,the level of MMP-3 was also different,especially betweenⅠandⅡ,and Ⅲ(P< 0.05).Conclusion Though there is individual difference,MMP-3 can be used as an indicator of disease activity.In the stage of X-Ⅰto Ⅱ, the MMP-3 level increases obviously.This suggests that high serum MMP-3 level indicates cartilage breakdoum and early intervention should be initiated.
出处
《中华风湿病学杂志》
CAS
CSCD
2004年第1期16-19,共4页
Chinese Journal of Rheumatology
