摘要
目的:掌握我国北方地区乙型肝炎病毒(HBV)感染者HBV基因型分布情况,为了解其流行病学及临床意义提供基础. 方法:首先采用巢式PCR法对801份血清进行HBV DNA检测,其中包括154份健康人血清、594份HBV感染的各类肝病患者及53例抗HCV阳性血清标本.再采用HBV A- F六个主要基因型特异性多引物对巢式PCR法对HBV DNA 进行基因型检测及分析. 结果:在801例血清标本中有464例(57.9%)检测到HBV DNA,其中154名健康人血清阳性率为8.4%(13/154), 594例不同临床表现的乙肝患者血清HBV DNA总检出率为74.4%(442/594),二者相比差异显著(P<0.01);抗HCV阳性血清中检出HBV DNA占17%(9/53).464例HBV DNA阳性标本中,HBV基因型检出率分别是:A型为5.2%(24/464), B型为7.1%(33/464),C型为77.2%(358/464),D型2.4% (11/464),未检出E和F型,但尚有14例(3%)同时检出B型和C型,有24例未检出型别.急性肝炎、慢性肝炎、肝硬化、原发肝癌各不同临床表现组间基因型分布无统计学差异. 结论:结果看出,我国北方地区感染的HBV基因型存在A、B、C、D四个型别,其中C型为主(77.2%),各临床表现组基因型分布也均以C型为主,组间无统计学差异.
AIM: To investigate the epidemiology of HBV genotypes among HBV-infected persons in northern China. METHODS: The HBV DNA in 801 sera samples collected from hepatitis patients (594), healthy controls (154), and HCV-infected patients (53) were tested by nested PCR, the positive sera were then genotyped by nested PCR with six pairs of HBV genotype-specific primers (A to F). RESULTS: 464 samples (57.9%) were HBV DNA positive among the 801 samples. Among 594 cases with various hepatitis B clinical manifestations, 74.4% (442/594) were HBV-DNA positive, while 8.4% (13/154) of healthy controls were HBV-DNA positive. There was significant difference between the hepatitis patients and healthy controls (P<0.01). 17% (9/53) of HCV-infected patients were HBV-DNA positive, too. Among the 464 HBV-DNA positive samples, the percentage of genotype A was 5.2% (24/464), genotype B 7.1% (33/464), and type C 77.2% (358/464), type D 2.4% (11/464), while none of type E and F had been found in those samples. 14 samples (3%) were both type B and C positive. The 24 samples could not be genotyped in this study. CONCLUSION: The HBV genotypes prevailed among HBV-infected persons in northern China are types A, B, C, and D, and the major genotype is type C (77.2%). Type C is also the major genotype among the clinical groups. There is no statistical difference between the groups.
出处
《世界华人消化杂志》
CAS
2004年第5期1073-1076,共4页
World Chinese Journal of Digestology
基金
日本健康科学基金资助项目
No.0109~~
