摘要
目的 :观察一氧化氮 (NO)和一氧化氮合酶抑制剂NG-硝基 -L -精氨酸 (L -NA)对LPS性肺损伤的影响。方法 :采用静脉注射脂多糖 (LPS)复制急性肺损伤大鼠模型。将 4 0只SD大鼠随机分为 5组 :空白对照组、LPS模型组、L -NA高剂量 (2 0mg/kg)、中剂量 (10mg/kg)、低剂量 (5mg/kg)治疗组 ,经腹腔注射 ,实验过程中监测大鼠平均动脉压 (MAP) ,定时取静脉血测定血浆中NO2 -/NO3 -含量 ,于规定时间处死大鼠 ,迅速取出肺脏 ,观察LPS引起大鼠急性肺损伤后肺系数、肺水肿情况和肺组织中丙二醛 (MDA)含量、一氧化氮合酶 (NOS)、超氧化物歧化酶(SOD)活性的变化 ,以及L -NA的治疗作用。结果 :L -NA可明显升高MAP ,降低肺系数和肺含水量 ,减少血浆中NO2 -/NO3 -含量 ,可显著降低肺组织中NOS活性 ,减少MDA含量 ,增强SOD活性 ,减轻肺损伤。结论 :L -NA对LPS性肺损伤具有治疗作用 ,且随剂量增大作用增强。
AIM: To investigate the effects of nitric oxide (NO) and NO synthase (NOS) inhibitor NG-nitro-L arginine (L-NA) on LPS induced-lung injury in rats. METHODS: Forty healthy male SD rats, weighing 300±20 g, were used. The animals were anesthetized with 20% urethane 1 g·kg -1. Common carotid artery (CAA) and jugular vein were exposed through a median incision in the neck. Mean arterial pressure (MAP) was measured through a pressure transducer connected with intubation of CAA. The animals were randomly divided into five groups: group 1: control; group 2: LPS (5 mg·kg -1, iv); group 3: high dose L-NA (20 mg·kg -1 intraperitoneal injection, ip); gropu 4: middle dose L-NA (10 mg·kg -1, ip); group 5: low dose L-NA (5 mg·kg -1, ip). Group1 : 0.9% saline solution was given and the animals were killed 6 h after the saline solution. Gruop 2: saline solution was given 3 h after LPS and the animals were killed 3 h after administration. Group 3, 4 and 5: L-NA was given 3 h after LPS iv and the animals were killed 3 h after administration, respectively. The pulmonary was removed immediately. The pulmonary coefficient and water content in pulmonary tissue were calculated (%). The NO 2-/NO 3- content in plasma, MDA content and NOS, SOD activity in the pulmonary tissue were measured. RESULTS: L-NA significantly decreased pulmonary coefficient and water content in pulmonary tissue and ameliorated LPS induced lung injury. The effect in high dose group was better than that in low dose group. L-NA significantly decreased NO 2-/NO 3- content in plasm, decreased MDA content and inhibited NOS activity and enhanced SOD activity in the pulmonary tissue. CONCLUSION: It may be concluded that L-NA has a beneficial effect on lung injury induced by LPS.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第5期769-773,共5页
Chinese Journal of Pathophysiology
基金
河北省博士基金资助项目 (No.995 4 70 15 )
关键词
肺损伤
急性
一氧化氮
精氨酸
脂多糖类
大鼠
Lung injury, acute
Nitric oxide
Arginine
Lipopolysaccharides
Rats
